Evidence map›Paper›PMID 33372687›Full record

ArticleHistology and histopathology2020

Diallyl trisulfide regulates cell apoptosis and invasion in human osteosarcoma U2OS cells through regulating PI3K/AKT/GSK3β signaling pathway.

Pan He, Zhijun Wang, Bin Sheng, Yongqiang Xu, Siyin Feng, Yan Huang, Fuqiang Gong, Liting Tang, Liming Xie

Abstract read
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In one paragraph

Article in Histology and histopathology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. NOTCH Signaling in Osteosarcoma.Current issues in molecular biology · 2023
    Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Pan HeThe Department of Traumatic and Osteopathology, Hunan provincial people's hospital, Changsha, Hunan, China. h13487490749@126.com.
Zhijun WangThe Department of Traumatic and Osteopathology, Hunan provincial people's hospital, Changsha, Hunan, China.
Bin ShengThe Department of Traumatic and Osteopathology, Hunan provincial people's hospital, Changsha, Hunan, China.
Yongqiang XuThe Department of Traumatic and Osteopathology, Hunan provincial people's hospital, Changsha, Hunan, China.
Siyin FengThe Department of Traumatic and Osteopathology, Hunan provincial people's hospital, Changsha, Hunan, China.
Yan HuangThe Department of Traumatic and Osteopathology, Hunan provincial people's hospital, Changsha, Hunan, China.
Fuqiang GongThe First Affiliated Hospital, University of South China, Hengyang, Hunan, China.
Liting TangThe First Affiliated Hospital, University of South China, Hengyang, Hunan, China.
Liming XieThe First Affiliated Hospital, University of South China, Hengyang, Hunan, China. xlmusc@163.com.
First Affiliated Hospital of University of South China · CNUniversity of South China · CN

Funding

Natural Science Foundation of Hunan Province, China 2020JJ4402Science and Technology Department of Hunan Province, China 2018JJ6071
6 · The paper itself

Abstract

aimsTo investigate the effects and the mechanisms of action of Diallyl trisulfide (DATS) on the proliferation and metastasis of human osteosarcoma (OS) U2OS.

methodsU2OS cells were treated by different concentrations of DATS at different time points. Cell proliferations were measured by MTT assay. DATS induced cell cycle distribution and apoptosis were evaluated by flow cytometry (FCM) with Annexin-V. Cell migration and invasion were detected by wound healing assay and transwell assay. The effects of DATS in U2OS cell growth and metastasis were also detected in a mouse OS xenograft model.

resultsA time- and concentration-dependent cytotoxic effect of DATS was observed in U2OS cells. FCM with PI staining and Annexin-V -FITC indicated that DATS induces apoptosis and a G0/G1 cell cycle arrest of U2OS cells at all concentrations from 25 μmol/l to 100 μmol/l. DATS also inhibits the migration and invasion of U2OS cells. Western blot showed that the expression levels of p-AKT, p-GSK3β, Bcl-2, Vimentin and β-catenin were decreased, while the expression levels of Bad, Bax and E-cadherin were significantly increased in DATS treated U2OS cells. Analysis using a mouse xenograft model indicated that xenografts of DATS treatment group had a significant decrease in tumor volume and weight compared to the control group. Lung metastasis models in mice demonstrated that treatment of DATS inhibits lung metastasis of OS in vivo.

conclusionsThese data suggested that DATS inhibits OS development and progression through the regulation of PI3K/AKT/GSK3β signaling pathways, accompanied by downregulation of Bcl-2, Vimentin and β-catenin, as well as upregulation of Bad, Bax and E-cadherin. Therefore, our data demonstrated that DATS exerted its anticancer effects by inhibiting cell proliferation, migration and invasion in vitro and in vivo. These results provide evidence for the use of the natural product DATS either alone or in combination with standard therapy for OS.

Indexed as

Allyl CompoundsAnimalsAntineoplastic AgentsApoptosisApoptosis Regulatory ProteinsBone NeoplasmsCell Cycle CheckpointsCell Line, TumorCell MovementCell ProliferationGlycogen Synthase Kinase 3 betaHumansMaleMice, Inbred BALB CMice, NudeNeoplasm InvasivenessAllyl CompoundsAntineoplastic AgentsApoptosis Regulatory Proteinsdiallyl trisulfideGlycogen Synthase Kinase 3 betaGSK3B protein, humanPhosphatidylinositol 3-KinaseProto-Oncogene Proteins c-aktSulfides

Identifiers

PMID33372687
OpenAlexW3113913241

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.