ArticleInsects2020
Non-Destructive Genotyping of Honeybee Queens to Support Selection and Breeding.
Article in Insects, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Comparing Morphometric and Mitochondrial DNA Data from Honeybees and Honey Samples for IdentifyingAnimals : an open access journal from MDPI · 2025Article
- Population-wide modelling reveals prospects of marker-assisted selection for parasitic mite resistance in honey bees.Scientific reports · 2024Article
- Identification of runs of homozygosity in Western honey bees (Ecology and evolution · 2023Article
- Cutting corners: The impact of storage and DNA extraction on quality and quantity of DNA in honeybee (Frontiers in physiology · 2023Article
- Reconstructing queen genotypes by pool sequencing colonies in eusocial insects: Statistical Methods and their application to honeybee.Molecular ecology resources · 2022Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In traditional bee breeding, the honeybee queen is chosen for breeding based on the performance of the colony produced by its mother. However, we cannot be entirely certain that a specific queen will produce offspring with desirable traits until we observe the young queen's new colony. Collecting the queen's genetic material enables quick and reliable determination of the relevant information. We sampled exuviae, feces, and wingtips for DNA extraction to avoid fatally injuring the queen when using tissue samples. Quantity and purity of extracted DNA were measured. Two mitochondrial markers were used to determine the lineage affiliation and exclude possible contamination of DNA extracts with non-honeybee DNA. dCAPS (derived Cleaved Amplified Polymorphic Sequences) markers allowed detection of single nucleotide polymorphisms (SNPs) in nuclear DNA regions presumably associated with Varroa sensitive hygiene and set the example of successful development of genotyping protocol from non-destructive DNA sources. One of the logical future steps in honeybee breeding is introducing genomic selection and non-destructive sampling methods of genetic material may be the prerequisite for successful genotyping. Our results demonstrate that the extraction of DNA from feces and exuviae can be introduced into practice. The advantage of these two sources over wingtips is reducing the time window for processing the samples, thus enabling genotyping directly after the queen's emergence.
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Registered trials
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