ArticlePLoS genetics2020
MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA.
Article in PLoS genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- RodZ acts through MreBCD to activate the elongasome inJournal of bacteriology · 2026Article
- Direct interaction between MreB and the RodA-PBP2 complex organizes lateral cell wall synthesis inmLife · 2026Article
- Twin-arginine transport complex plays an essential role inMolecular biology of the cell · 2026Article
- Alanine-scanning mutagenesis library of MreB reveals distinct roles for regulating cell shape and viability.PLoS genetics · 2026Article
- The roles of theJournal of bacteriology · 2026Article
- CoccoidVirulence · 2025Review
- Growth stage and interspecies interactions shape the cell biology and cell cycle characteristics of human gut bacteria Bacteroides thetaiotaomicron and Roseburia intestinalis.Communications biology · 2025Article
- Rapid key gene discovery for bacterial shape: a cross-species machine learning approach.BMC microbiology · 2025Article
- Twin-arginine transport complex plays an essential role inbioRxiv : the preprint server for biology · 2025Article
- Peptidoglycan polymerase function and regulation.Microbiology and molecular biology reviews : MMBR · 2025Review
- RagB stimulates the activity of the peptidoglycan polymerase RodA in Bacillus subtilis.EMBO reports · 2025Article
- Article
- A nucleus-encoded dynamin-like protein controls endosymbiont division in the trypanosomatidScience advances · 2025Article
- Characterization of MreCD inJournal of oral microbiology · 2025Article
- Peptidoglycan Endopeptidase PBP7 Facilitates the Recruitment of FtsN to the Divisome and Promotes Peptidoglycan Synthesis in Escherichia coli.Molecular microbiology · 2024Article
- DnaA modulates the gene expression and morphology of the Lyme disease spirochete.bioRxiv : the preprint server for biology · 2024Article
- The role of GpsB inmBio · 2024Article
- A mobile CRISPRi collection enables genetic interaction studies for the essential genes of Escherichia coli.Cell reports methods · 2024Article
- Article
- Comprehensive understanding of the mutant 'giant'Frontiers in plant science · 2024Article
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5 authors.
Funding
Abstract
Rod-shape of most bacteria is maintained by the elongasome, which mediates the synthesis and insertion of peptidoglycan into the cylindrical part of the cell wall. The elongasome contains several essential proteins, such as RodA, PBP2, and the MreBCD proteins, but how its activities are regulated remains poorly understood. Using E. coli as a model system, we investigated the interactions between core elongasome proteins in vivo. Our results show that PBP2 and RodA form a complex mediated by their transmembrane and periplasmic parts and independent of their catalytic activity. MreC and MreD also interact directly with PBP2. MreC elicits a change in the interaction between PBP2 and RodA, which is suppressed by MreD. The cytoplasmic domain of PBP2 is required for this suppression. We hypothesize that the in vivo measured PBP2-RodA interaction change induced by MreC corresponds to the conformational change in PBP2 as observed in the MreC-PBP2 crystal structure, which was suggested to be the "on state" of PBP2. Our results indicate that the balance between MreC and MreD determines the activity of PBP2, which could open new strategies for antibiotic drug development.
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