Evidence map›Paper›PMID 33368048›Full record

ArticleDoklady. Biochemistry and biophysics2020

Novel pH-Sensitive Microbial Rhodopsin from Sphingomonas paucimobilis.

N Maliar, I S Okhrimenko, L E Petrovskaya, A A Alekseev, K V Kovalev, D V Soloviov, P A Popov, T I Rokitskaya, Y N Antonenko, D V Zabelskii and 3 more

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Doklady. Biochemistry and biophysics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Article
  3. Mirror proteorhodopsins.Communications chemistry · 2023
    Article
  4. Article
  5. Article
  6. Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  7. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 7 institutions in 4 countries.

N MaliarMoscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia.
I S OkhrimenkoMoscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia.
L E PetrovskayaShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
A A AlekseevMoscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia.
K V KovalevMoscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia.
D V SoloviovMoscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia.
P A PopovMoscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia.
T I RokitskayaBelozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia.
Y N AntonenkoBelozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, Russia.
D V ZabelskiiMoscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia.
D A DolgikhShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
M P KirpichnikovShemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
V I GordeliyMoscow Institute of Physics and Technology, Dolgoprudnyi, Moscow oblast, Russia. valentin.gordeliy@ibs.fr.
Moscow Institute of Physics and Technology · RULomonosov Moscow State University · RUInstitute of Bioorganic Chemistry · RUCentre National de la Recherche Scientifique · FRCommissariat à l'Énergie Atomique et aux Énergies Alternatives · FRForschungszentrum Jülich · DENational Academy of Sciences of Ukraine · UA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This work provides the first characteristics of the rhodopsin SpaR from Sphingomonas paucimobilis, aerobic bacteria associated with opportunistic infections. The sequence analysis of SpaR has shown that this protein has unusual DTS motif which has never reported in rhodopsins from Proteobacteria. We report that SpaR operates as an outward proton pump at low pH; however, proton pumping is almost absent at neutral and alkaline pH. The photocycle of this rhodopsin in detergent micelles slows down with an increase in pH because of longer Schiff base reprotonation. Our results show that the novel microbial ion transporter SpaR of interest both as an object for basic research of membrane proteins and as a promising optogenetic tool.

Indexed as

Hydrogen-Ion ConcentrationLightOptogeneticsProton PumpsRhodopsinRhodopsins, MicrobialSphingomonasProton PumpsRhodopsinRhodopsins, Microbiallight-activated ion transporterlight-driven proton pumpmicrobial rhodopsinoptogenetics

Identifiers

PMID33368048
OpenAlexW3113680260

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.