Evidence map›Paper›PMID 33368010›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Detection of Microcrystals for CryoEM.

Simon Weiss, Sandra Vergara, Guowu Lin, Guillermo Calero

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Applying 3D ED/MicroED workflows toward the next frontiers.Acta crystallographica. Section C, Structural chemistry · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Simon WeissDepartment of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Sandra VergaraDepartment of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Guowu LinDepartment of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Guillermo CaleroDepartment of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA. guc9@pitt.edu.

Funding

X-Ray Core P50GM082251 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI CALERO, GUILLERMO ALBERTO · 2007 to 2018
$48.1M
Technology Development 2: MAS NMR and dynamic nuclear polarization for HIV-1 structural biologyP50AI150481 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI GRONENBORN, ANGELA M. · 2019 to 2021
$14.1M
Structural Studies of RNA Polymerase II Transcription Initiation and ElongationR01GM112686 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI CALERO, GUILLERMO ALBERTO · 2015 to 2024
$2.8M
Real-time discovery of inhibitors among billion compounds for preview and downloadR01GM097082 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI CAMACHO, CARLOS J. · 2011 to 2020
$2.5M
NIAID NIH HHS P50 AI150481NIGMS NIH HHS P50 GM082251NIGMS NIH HHS R01 GM097082NIGMS NIH HHS R01 GM112686
6 · The paper itself

Abstract

Here, we present a strategy to identify microcrystals from initial protein crystallization screen experiments and to optimize diffraction quality of those crystals using negative stain transmission electron microscopy (TEM) as a guiding technique. The use of negative stain TEM allows visualization along the process and thus enables optimization of crystal diffraction by monitoring the lattice quality of crystallization conditions. Nanocrystals bearing perfect lattices are seeded and can be used for MicroED as well as growing larger crystals for X-ray and free electron laser (FEL) data collection.

Indexed as

Protein ConformationCryoelectron MicroscopyCrystallizationMicroscopy, Electron, TransmissionNanoparticlesBrightfield microscopyCrystal lattice qualityFFT-calculationGranular aggregateMicrocrystalNegative staining TEMOptimizationProtein crystallizationUV microscopy

Identifiers

PMID33368010
PMCPMC8188421

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.