ArticleMethods in molecular biology (Clifton, N.J.)2021
In Situ Imaging and Structure Determination of Biomolecular Complexes Using Electron Cryo-Tomography.
Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- A practical guide to investigating biomolecular condensates: a comment from the plant community.Science China. Life sciences · 2026Review
- Bacterial nanotubes extend from a tubeosome organelle to establish an intercellular bridge.bioRxiv : the preprint server for biology · 2026Article
- Distinct motors, shared mechanics: unifying principles of microbial gliding.Journal of bacteriology · 2026Review
- A robust workflow for 3D imaging of human mitochondria using cryo-electron tomography.bioRxiv : the preprint server for biology · 2026Article
- Diel remodeling and cellular integration of the nitroplast.bioRxiv : the preprint server for biology · 2026Article
- Automated removal of corrupted tilts in cryo-electron tomography.Journal of structural biology: X · 2025Article
- Large attachment organelle mediates interaction between Nanobdellota archaeon YN1 and its host.The ISME journal · 2024Article
- Structure and Assembly of theInternational journal of molecular sciences · 2023Article
- Discovery of a Novel Inner Membrane-Associated Bacterial Structure Related to the Flagellar Type III Secretion System.Journal of bacteriology · 2022Article
- Better, Faster, Cheaper: Recent Advances in Cryo-Electron Microscopy.Annual review of biochemistry · 2022Review
- High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity.Nature protocols · 2022Review
- Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Electron cryo-tomography (cryo-ET) is a technique that allows the investigation of intact macromolecular complexes while they are in their cellular milieu. Over the years, cryo-ET has had a huge impact on our understanding of how large biomolecular complexes look like, how they assemble, disassemble, function, and evolve(d). Recent hardware and software developments and combining cryo-ET with other techniques, e.g., focused ion beam milling (FIB-milling) and cryo-light microscopy, has extended the realm of cryo-ET to include transient molecular complexes embedded deep in thick samples (like eukaryotic cells) and enhanced the resolution of structures obtained by cryo-ET. In this chapter, we will present an outline of how to perform cryo-ET studies on a wide variety of biological samples including prokaryotic and eukaryotic cells and biological plant tissues. This outline will include sample preparation, data collection, and data processing as well as hybrid approaches like FIB-milling, cryosectioning, and cryo-correlated light and electron microscopy (cryo-CLEM).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.