ArticleNucleic acids research2021
Differential activation mechanisms of two isoforms of Gcr1 transcription factor generated from spliced and un-spliced transcripts in Saccharomyces cerevisiae.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed, 14 citations in OpenAlex.
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- Intron Retention Controls Localization of lncRNAsbioRxiv : the preprint server for biology · 2026Article
- Regulating the metabolic flux of pyruvate dehydrogenase bypass to enhance lipid production in Saccharomyces cerevisiae.Communications biology · 2024Article
- Review
- Mechanisms and biotechnological applications of transcription factors.Synthetic and systems biotechnology · 2023Review
- A New Mechanism of Carbon Metabolism and Acetic Acid Balance Regulated by CcpA.Microorganisms · 2023Article
- Rewiring regulation on respiro-fermentative metabolism relieved Crabtree effects inSynthetic and systems biotechnology · 2022Article
- Transcriptomic Changes Induced by Deletion of Transcriptional RegulatorFrontiers in bioengineering and biotechnology · 2021Article
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Authors and funding
4 authors at 3 institutions in 1 country.
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Abstract
Gcr1, an important transcription factor for glycolytic genes in Saccharomyces cerevisiae, was recently revealed to have two isoforms, Gcr1U and Gcr1S, produced from un-spliced and spliced transcripts, respectively. In this study, by generating strains expressing only Gcr1U or Gcr1S using the CRISPR/Cas9 system, we elucidate differential activation mechanisms of these two isoforms. The Gcr1U monomer forms an active complex with its coactivator Gcr2 homodimer, whereas Gcr1S acts as a homodimer without Gcr2. The USS domain, 55 residues at the N-terminus existing only in Gcr1U, inhibits dimerization of Gcr1U and even acts in trans to inhibit Gcr1S dimerization. The Gcr1S monomer inhibits the metabolic switch from fermentation to respiration by directly binding to the ALD4 promoter, which can be restored by overexpression of the ALD4 gene, encoding a mitochondrial aldehyde dehydrogenase required for ethanol utilization. Gcr1U and Gcr1S regulate almost the same target genes, but show unique activities depending on growth phase, suggesting that these isoforms play differential roles through separate activation mechanisms depending on environmental conditions.
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