Evidence map›Paper›PMID 33364625›Full record

ArticleSTAR protocols2021

An optimized protocol for combined fluorescent lectin/immunohistochemistry to characterize tissue-specific glycan distribution in human or rodent tissues.

Ana Lúcia Rebelo, Paolo Contessotto, Kieran Joyce, Michelle Kilcoyne, Abhay Pandit

Abstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
  7. Changes in tissue proteinPNAS nexus · 2024
    Article
  8. Article
  9. N-glycans show distinct spatial distribution in mouse brain.bioRxiv : the preprint server for biology · 2023
    Article
  10. Article
  11. Update on Perineuronal Net Staining WithFrontiers in integrative neuroscience · 2022
    Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ana Lúcia RebeloCÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.
Paolo ContessottoCÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.
Kieran JoyceCÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.
Michelle KilcoyneCarbohydrate Signalling Group, Microbiology, School of Natural Sciences, National University of Ireland Galway, Galway H92 W2TY, Ireland.
Abhay PanditCÚRAM SFI Research Centre for Medical Devices, National University of Ireland Galway, Galway H92 W2TY, Ireland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lectin histochemical analysis of tissues combined with immunohistochemistry is a valuable tool to characterize and correlate the spatial distribution of glycans with the presence of specific cell types or antigens of interest. The current protocol describes the application of monosaccharide motif specificity of lectin binding to glycan residues to different tissue types. In addition, we describe stereological methods to provide further quantification of the analyzed tissues. For complete details on the use and execution of this protocol, please refer to Mohd Isa et al. (2018), Contessotto et al. (2020), and Samal et al. (2020).

Indexed as

ImmunohistochemistryAnimalsFluorescent DyesHumansLectinsMiceOrgan SpecificityPolysaccharidesFluorescent DyesLectinsPolysaccharidesGlycobiologyMicroscopyMolecular BiologyTissue Engineering

Identifiers

PMID33364625
PMCPMC7753946

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.