Evidence map›Paper›PMID 33364621›Full record

ArticleSTAR protocols2021

sncRiboTag-Seq: cell-type-specific RiboTag-Seq for cells in low abundance in mouse brain tissue.

Yu Gao, Xinyu Zhao

Open access · goldAbstract read
In one paragraph

Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
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  3. Review
  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Yu GaoWaisman Center, University of Wisconsin-Madison, Madison, WI 53705, USA.
Xinyu ZhaoWaisman Center, University of Wisconsin-Madison, Madison, WI 53705, USA.
University of Wisconsin–Madison · US

Funding

Waisman Intellectual and Developmental Disabilities Research Center Down Syndrome RegistryU54HD090256 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI POLLAK, SETH D · 2016 to 2020
$6.0M
The Role of MDM2 in FMRP regulation of neuronal developmentR01MH116582 · NIMH · UNIVERSITY OF WISCONSIN-MADISON · PI ZHAO, XINYU · 2018 to 2022
$2.2M
The role of RNA binding protein in FXR1P in interneuronsR01MH118827 · NIMH · UNIVERSITY OF WISCONSIN-MADISON · PI ZHAO, XINYU · 2019 to 2023
$2.2M
Integration of Experience-Induced Gene Expression and Circuit FunctionsR01NS105200 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI JACKSON, MEYER B., ZHAO, XINYU · 2018 to 2022
$2.0M
Coordinate actions between methyl-CpG binding proteins in neuronal developmentR21NS095632 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI ZHAO, XINYU · 2015 to 2016
$418k
Circuit Activity and Synaptic Integration of Newborn Neurons in the Dentate GyrusR21NS098767 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI JACKSON, MEYER B., ZHAO, XINYU · 2017 to 2018
$408k
Epigenetic Crosstalk Regulates Neuronal DevelopmentR56MH113146 · NIMH · UNIVERSITY OF WISCONSIN-MADISON · PI ZHAO, XINYU · 2017 to 2017
$383k
NICHD NIH HHS U54 HD090256NIMH NIH HHS R01 MH116582NIMH NIH HHS R01 MH118827NIMH NIH HHS R56 MH113146NINDS NIH HHS R01 NS105200NINDS NIH HHS R21 NS095632NINDS NIH HHS R21 NS098767
6 · The paper itself

Abstract

The mammalian brain has over 10,000 types of neurons. Therefore, studying gene regulation in the brain requires effective strategies for targeting specific cell types, especially those in low abundance. Cell isolation may alter gene expression and is disruptive to mature neurons with extensive processes. This protocol describes cell-type-specific expression of tagged ribosome and the use of ribosome tagging followed by RNA-seq to identify translatome of low number and sparse cells in mouse brains without disruptive cell isolation. For complete details on the use and execution of this protocol, please refer to Gao et al. (2020).

Indexed as

RNA, MessengerRNA-SeqAnimalsBrainMiceNeuronsRNA, MessengerGene expressionNeuroscienceRNA-seq

Identifiers

PMID33364621
PMCPMC7753947
OpenAlexW3114458223

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.