Evidence map›Paper›PMID 33362748›Full record

ArticleFrontiers in microbiology2020

Loop-Mediated Isothermal Amplification (LAMP) as a Rapid, Affordable and Effective Tool to Involve Students in Undergraduate Research.

Andrew V Nguyen, Amos Orlofsky, Kaylynn Pubill, Mangala Tawde, Gaozhen Li, Diana Mata, Oscar Bermudes, Miguel Fernandez, Jonathan Santana, Woochul Kim and 9 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 2 pooled it
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 2 syntheses or guidelines pooled it, 12 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
  4. Article
  5. Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 6 institutions in 1 country.

Andrew V NguyenDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Amos OrlofskyDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Kaylynn PubillDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Mangala TawdeDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Gaozhen LiDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Diana MataDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Oscar BermudesDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Miguel FernandezDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Jonathan SantanaDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Woochul KimDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Enzon ChimbayDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Yeeun KimDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Trieu NguyenDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Malcolm FoxDepartment of Biological Sciences and Geology, The City University of New York, Queensborough Community College, Bayside, NY, United States.
Janelly EralteDepartment of Natural Sciences, Mercy College, Dobbs Ferry, NY, United States.
Molly MetzDepartment of Natural Sciences and Mathematics, Eugene Lang College of Liberal Arts of the New School, New York, NY, United States.
Davida S SmythDepartment of Natural Sciences and Mathematics, Eugene Lang College of Liberal Arts of the New School, New York, NY, United States.
Caterina PanzecaDepartment of Science, SUNY Maritime College, New York, NY, United States.
Mazhar I KhanDepartment of Pathobiology and Veterinary Science, College of Agriculture, Health and Natural Resources, Storrs, CT, United States.
City University of New York · USQueensborough Community College, CUNY · USNew School · USMercy University · USSUNY Maritime College · USUniversity of Connecticut · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Undergraduate research (UR) is a high-impact practice (HIP) to engage undergraduate student in science, technology, engineering and mathematics (STEM), especially from underrepresented groups. UR experiences (UREs) can be integrated into the classroom, making authentic research experiences inclusive and available to all students. However, developing UR pedagogy can be challenging for faculty in resource-limited labs, such as community colleges and small liberal arts colleges. Often molecular biology research methods are expensive, time-consuming and need equipment not readily available or affordable in small schools. Polymerase chain reaction (PCR) is one of the most commonly used techniques in research labs and many UREs. We have investigated loop-mediated isothermal amplification (LAMP) as an inexpensive, accessible alternative to PCR for DNA amplification enabling the identification of microorganisms in the context of UREs. LAMP does not require expensive instrumentation or reagents and uses equipment commonly found in teaching labs. By performing the technique, students learn several key scientific skills that will be useful in their undergraduate or graduate STEM careers. We designed guided independent research experiences for several undergraduates that included the use of LAMP. Students successfully applied the technique to culture samples of common environmental bacteria, including

Indexed as

HIP’sLAMPresearch toolstem educationundergraduate research (UR)

Identifiers

PMID33362748
PMCPMC7756096
OpenAlexW3111663873

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.