Trial reportPloS one2020
Assessment of the sublingual microcirculation with the GlycoCheck system: Reproducibility and examination conditions.
Trial report in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Biomarkers of endothelial glycocalyx dysfunction in pregnancy: a systematic review of clinical relevance and detection techniques.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026Pooled it
- Impact of dietary supplementation of glycocalyx precursors on vascular function in type 2 diabetes.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Trial
- Effect of glycocalyx-targeted therapy on vascular function in older adults: a randomized controlled trial.Journal of applied physiology (Bethesda, Md. : 1985) · 2024Trial
- Trial
- Observational
- Evaluating the endothelial glycocalyx from bench to bedside: a translational framework for microvascular assessment.Angiogenesis · 2026Review
- Endothelial-erythrocyte glycocalyx exchange enables liquid biopsies of endothelial function.Nature communications · 2026Article
- Assessing Microcirculation in Heart Failure: No Sweet Without Sweat.JACC. Advances · 2026Article
- Early detection of glycocalyx and microvascular damage in suspected sepsis in the emergency department: the EDGE study.Critical care (London, England) · 2026Observational
- International Utah Cardiac Recovery Symposium (U-CARS) 2025: Clinical Science Proceedings.Heart failure reviews · 2026Article
- Endothelial glycocalyx thickness in cats with naturally occurring trauma or non-traumatic illness: an exploratory study.Frontiers in veterinary science · 2026Article
- Glycosaminoglycans and Their Role in Capillary Walls.Advances in experimental medicine and biology · 2026Review
- Sublingual microcirculation-derived glycocalyx barrier impairment independently predicts progression of asymptomatic left ventricular diastolic dysfunction in elderly Chinese adults.Frontiers in cardiovascular medicine · 2026Article
- Reproducibility of glycocheck measurements in patients under general anesthesia with muscle relaxants: A prospective observational study.Journal of clinical monitoring and computing · 2025Observational
- Sugar-coating the endothelium: adaptive or maladaptive?American journal of physiology. Heart and circulatory physiology · 2025Article
- Endothelial Glycocalyx Anomalies and Ocular Manifestations in Patients with Post-Acute COVID-19.Journal of clinical medicine · 2024Article
- Association of Gestational Diabetes With Subclinical Cardiovascular Disease.JACC. Advances · 2024Article
- Doxycycline protects against sepsis-induced endothelial glycocalyx shedding.Scientific reports · 2024Article
- High salt intake and HIV infection on endothelial glycocalyx shedding in salt-sensitive hypertension.Frontiers in cell and developmental biology · 2024Review
- The future of intensive care: the study of the microcirculation will help to guide our therapies.Critical care (London, England) · 2023Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe glycocalyx is an extracellular layer lining the lumen of the vascular endothelium, protecting the endothelium from shear stress and atherosclerosis and contributes to coagulation, immune response and microvascular perfusion. The GlycoCheck system estimates glycocalyx' thickness in vessels under the tongue from perfused boundary region (PBR) and microvascular perfusion (red blood cell (RBC) filling) via a camera and dedicated software.
objectivesEvaluating reproducibility and influence of examination conditions on measurements with the GlycoCheck system.
methodsOpen, randomised, controlled study including 42 healthy smokers investigating day-to-day, side-of-tongue, inter-investigator variance, intraclass-correlation (ICC) and influence of examination conditions at intervals from 0-180 minutes on PBR and RBC filling.
resultsMean (SD) age was 24.9 (6.1) years, 52% were male. There was no significant intra- or inter-investigator variation for PBR or RBC filling nor for PBR for side-of-tongue. A small day-to-day variance was found for PBR (0.012μm, p = 0.007) and RBC filling (0.003%, p = 0.005) and side-of-tongue, RBC filling (0.025%, p = 0.009). ICC was modest but highly improved by increasing measurements. Small significant influence of cigarette smoking (from 40-180 minutes), high calorie meal intake and coffee consumption was found. The latter two peaking immediately and tapering off but remained significant up to 180 minutes, highest PBR changes for the three being 0.042μm (p<0.05), 0.183μm (p<0.001) and 0.160μm (p<0.05) respectively.
conclusionsMeasurements with the GlycoCheck system have a moderate reproducibility, but highly increases with multiple measurements and a small day-to-day variability. Smoking, meal and coffee intake had effects up to 180 minutes, abstinence is recommended at least 180 minutes before GlycoCheck measurements. Future studies should standardise conditions during measurements.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.