Evidence map›Paper›PMID 33361597›Full record

ArticleJournal of Alzheimer's disease : JAD2021

Behavioral Deficits and Brain α-Synuclein and Phosphorylated Serine-129 α-Synuclein in Male and Female Mice Overexpressing Human α-Synuclein.

Lilit Gabrielyan, Honghui Liang, Artem Minalyan, Asa Hatami, Varghese John, Lixin Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of Alzheimer's disease : JAD, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.4field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Lilit GabrielyanCURE/Digestive Disease Research Center, Med/Digestive, David Geffen Medical School, UCLA, Los Angeles, CA, USA.
Honghui LiangCURE/Digestive Disease Research Center, Med/Digestive, David Geffen Medical School, UCLA, Los Angeles, CA, USA.
Artem MinalyanCURE/Digestive Disease Research Center, Med/Digestive, David Geffen Medical School, UCLA, Los Angeles, CA, USA.
Asa HatamiDrug Discovery Lab, Department of Neurology, UCLA, Los Angeles, CA, USA.
Varghese JohnDrug Discovery Lab, Department of Neurology, UCLA, Los Angeles, CA, USA.
Lixin WangCURE/Digestive Disease Research Center, Med/Digestive, David Geffen Medical School, UCLA, Los Angeles, CA, USA.
Digestive Care (United States) · US

Funding

Pilot and Feasibility ProgramP30DK041301 · NIDDK · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ROZENGURT, JUAN ENRIQUE · 1990 to 2019
$18.0M
Comprehensive Structural and Functional Mapping of Mammalian Colonic Nervous SystemOT2OD024899 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI MULUGETA, MILLION, TACHE, YVETTE FRANCE · 2017 to 2021
$13.1M
Ghrelin Agonists Multiple Beneficial Effects on Parkinson's Non-Motor SymptomsI01RX001685 · VA · VA GREATER LOS ANGELES HEALTHCARE SYSTEM · PI WANG, LIXIN · 2015 to 2020
–
NIDDK NIH HHS P30 DK041301NIH HHS OT2 OD024899RRD VA I01 RX001685
6 · The paper itself

Abstract

backgroundAlpha-synuclein (α-syn) is involved in pathology of Parkinson's disease, and 90% of α-syn in Lewy bodies is phosphorylated at serine 129 (pS129 α-syn).

objectiveTo assess behavior impairments and brain levels of α-syn and pS129 α-syn in mice overexpressing human α-syn under Thy1 promoter (Thy1-α-syn) and wild type (wt) littermates.

methodsMotor and non-motor behaviors were monitored, brain human α-syn levels measured by ELISA, and α-syn and pS129 α-syn mapped by immunohistochemistry.

resultsMale and female wt littermates did not show differences in the behavioral tests. Male Thy1-α-syn mice displayed more severe impairments than female counterparts in cotton nesting, pole tests, adhesive removal, finding buried food, and marble burying. Concentrations of human α-syn in the olfactory regions, cortex, nigrostriatal system, and dorsal medulla were significantly increased in Thy1-α-syn mice, higher in males than females. Immunoreactivity of α-syn was not simply increased in Thy1-α-syn mice but had altered localization in somas and fibers in a few brain areas. Abundant pS129 α-syn existed in many brain areas of Thy1-α-syn mice, while there was none or only a small amount in a few brain regions of wt mice. The substantia nigra, olfactory regions, amygdala, lateral parabrachial nucleus, and dorsal vagal complex displayed different distribution patterns between wt and transgenic mice, but not between sexes.

conclusionThe severer abnormal behaviors in male than female Thy1-α-syn mice may be related to higher brain levels of human α-syn, in the absence of sex differences in the altered brain immunoreactivity patterns of α-syn and pS129 α-syn.

Indexed as

alpha-SynucleinAnimalsBehavior, AnimalBrainBrain ChemistryEnzyme-Linked Immunosorbent AssayFemaleHumansMaleMiceMice, Inbred C57BLMice, TransgenicPhosphorylationSerineSex Factorsalpha-SynucleinSerineSNCA protein, humanbehaviorbrainmousephosphorylated serine-129 α-synucleinsynucleinopathyα-synuclein

Identifiers

PMID33361597
PMCPMC8577576
OpenAlexW3115234418

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.