Evidence map›Paper›PMID 33361423›Full record

ArticleJournal of virology2021

Differential Splicing of Human Adenovirus 5 E1A RNA Expressed in

Drayson Graves, Nikolas Akkerman, Scott Bachus, Peter Pelka

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Drayson GravesDepartment of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
Nikolas Akkerman *Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
Scott Bachus *Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
Peter PelkaDepartment of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada peter.pelka@umanitoba.ca.ORCID 0000-0003-0099-0422
University of Manitoba · CA

Funding

CIHR PJT-166198CIHR PJT-173376
6 · The paper itself

Abstract

Human adenovirus (HAdV) is used extensively as a vector for gene delivery for a variety of purposes, including gene therapy and vaccine development. Most adenoviral vectors used for these approaches have a deletion of early region 1 (E1), which is complemented by the cell line. Most commonly, these are 293 cells for HAdV serotype 2 or 5. The 293 cells have the left end of HAdV5 integrated into chromosome 19 and express the E1 genes and protein IX. We observed that viruses with the E1 region deleted often grow less well on 293 cells than E1 wild-type viruses. Therefore, we investigated whether this poor growth is caused by splicing differences between the E1A RNA provided by the cell line (in

Indexed as

Adenovirus E1A ProteinsAdenoviruses, HumanGene Expression Regulation, ViralGenetic Complementation TestHEK293 CellsHumansMutationRNA IsoformsRNA, MessengerRNA SplicingViral Replication CompartmentsVirus ReplicationAdenovirus E1A ProteinsRNA IsoformsRNA, Messenger293 cellsadenovirusE1Asplicing

Identifiers

PMID33361423
PMCPMC8094950
OpenAlexW3113615586

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.