ArticleThe Science of the total environment2021
Efficient detection of SARS-CoV-2 RNA in the solid fraction of wastewater.
Article in The Science of the total environment, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers, 5 of them syntheses that pooled it.
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Who cites it
73 citing papers in PubMed, 5 syntheses or guidelines pooled it.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Correlation between SARS-CoV-2 RNA concentration in wastewater and COVID-19 cases in community: A systematic review and meta-analysis.Journal of hazardous materials · 2023Pooled it
- Wastewater surveillance to infer COVID-19 transmission: A systematic review.The Science of the total environment · 2022Pooled it
- Data-driven estimation of COVID-19 community prevalence through wastewater-based epidemiology.The Science of the total environment · 2021Pooled it
- Measuring the efficiency of a solids-based wastewater extraction using BCoV and validation of a BCoV and PMMoV RT-qPCR duplex assay.MethodsX · 2026Article
- Assessing Urban Ditches for Tracking SARS-CoV-2 in Low Sanitation Areas in Southeastern Brazil.Food and environmental virology · 2026Article
- Optimizing solid-based methods for SARS-CoV-2 detection in wastewater: addressing PCR inhibition and variant challenges.Applied and environmental microbiology · 2025Article
- Solid Evidence and Liquid Gold: Trade-Offs of Processing Settled Solids, Whole Influent, or Centrifuged Influent for Codetecting Viral, Bacterial, and Eukaryotic Pathogens in Wastewater.Environmental science & technology · 2025Article
- Integrating wastewater surveillance for future pandemic preparedness by tracking SARS-CoV-2 RNA in municipal wastewater in Southwest Iran.Scientific reports · 2025Article
- Advances in Wastewater-Based Epidemiology for Pandemic Surveillance: Methodological Frameworks and Future Perspectives.Microorganisms · 2025Review
- Multisite community-scale monitoring of respiratory and enteric viruses in the effluent of a nursing home and in the inlet of the local wastewater treatment plant.Applied and environmental microbiology · 2024Article
- Article
- The first detection of SARS-CoV-2 RNA in the wastewater of Bucharest, Romania.Scientific reports · 2024Article
- Urban Wastewater-Based Surveillance of SARS-CoV-2 Virus: A Two-Year Study Conducted in City of Patras, Greece.Food and environmental virology · 2024Article
- Sewage surveillance of SARS-CoV-2 in Tokyo (May 2020-June 2022): method using high-throughput, fully-automated platform.Virusdisease · 2024Article
- Analytical validation of a semi-automated methodology for quantitative measurement of SARS-CoV-2 RNA in wastewater collected in northern New England.Microbiology spectrum · 2024Article
- Explaining the impact of mutations on quantification of SARS-CoV-2 in wastewater.Scientific reports · 2024Article
- A scoping review of global SARS-CoV-2 wastewater-based epidemiology in light of COVID-19 pandemic.Heliyon · 2024Article
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
13 more citing papers are in PubMed but not listed here.
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the context of the coronavirus disease 2019 (COVID-19) pandemic, environmental surveillance for the detection of SARS-CoV-2 has become increasingly important. Studies have demonstrated that the SARS-CoV-2 RNA is present in the feces of infected individuals; further, its presence in wastewater has been reported. However, an optimized method for its detection in sewage has not yet been adequately investigated. Therefore, in this study, the efficient detection of SARS-CoV-2 RNA in the solid fraction of wastewater was investigated using two quantitative PCR assays. In particular, wastewater samples were collected from a manhole located in the commercial district of a metropolitan region in Japan, where COVID-19 is highly prevalent, and two wastewater treatment plants (WWTPs). The samples were concentrated using four separate methods, namely, electronegative membrane adsorption, polyethylene glycol precipitation, ultrafiltration, and solid precipitation. Each method revealed a significant concentration of pepper mild mottle virus (PMMoV) RNA, which is an indicator virus for wastewater. As expected, non-enveloped PMMoV RNA was enriched in the supernatant fraction such that relatively low concentrations were detected in the solid fraction of the wastewater samples. In contrast, higher SARS-CoV-2 RNA concentrations were consistently detected in the solid fractions compared with the supernatant fractions based on the other methods that were investigated in this study. Spearman's correlation tests showed that the SARS-CoV-2 RNA concentrations in wastewater samples from the WWTP were significantly correlated with the number of COVID-19 cases recorded during the data collection period. These results demonstrate that viral recovery from the solid fraction is an effective method for SARS-CoV-2 RNA surveillance in an aqueous environment.
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