ArticleJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc2021
Implementation of next-generation sequencing for virus identification in veterinary diagnostic laboratories.
Article in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- Coinfection with Different Feline Papillomaviruses in a Cat with Multicentric Squamous Cell Carcinoma In Situ.Animals : an open access journal from MDPI · 2026Article
- Breaking the culture habit: Complementing culture-based veterinary diagnostics with metagenomic data -A case study of feline and canine skin infections.BMC veterinary research · 2026Article
- Combined NGS and proteomics improves viral diagnostics in wildlife: coronaviruses confirmed in hedgehogs.Frontiers in cellular and infection microbiology · 2026Article
- Advancements in Modern Nucleic Acid-Based Multiplex Testing Methodologies for the Diagnosis of Swine Infectious Diseases.Veterinary sciences · 2025Review
- Molecular characterization of ten viral pathogens causing calf diarrhea in Hanwoo (Bos Taurus coreanae) by next generation sequencing.BMC veterinary research · 2025Article
- Epidemiological, serological, and viral genomic analysis of an outbreak of elephant hemorrhagic disease in Switzerland.PloS one · 2025Article
- An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material.Microbiology spectrum · 2024Article
- Influenza surveillance in pigs: balancing act between broad diagnostic coverage and specific virus characterization.Porcine health management · 2024Article
- Investigation of laboratory methods for characterization of aquatic viruses in fish infected experimentally with infectious salmon anemia virus.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- The Impact of Pathogens on Sepsis Prevalence and Outcome.Pathogens (Basel, Switzerland) · 2024Review
- Rapid detection of high consequence and emerging viral pathogens in pigs.Frontiers in veterinary science · 2024Article
- Genome characteristics of atypical porcine pestivirus from abortion cases in Shandong Province, China.Virology journal · 2023Article
- Genome Sequence of Alongshan Virus from Ixodes ricinus Ticks Collected in Switzerland.Microbiology resource announcements · 2023Article
- Molecular genotyping, histopathological and immunohistochemical studies of bovine papillomatosis.Open veterinary journal · 2023Article
- Virus Diversity, Abundance, and Evolution in Three Different Bat Colonies in Switzerland.Viruses · 2022Article
- Differential Viral Genome Diversity of Healthy and RSS-Affected Broiler Flocks.Microorganisms · 2022Article
- Frequent Occurrence of Simultaneous Infection with Multiple Rotaviruses in Swiss Pigs.Viruses · 2022Article
- Exo-erythrocytic development of Plasmodium matutinum (lineage pLINN1) in a naturally infected roadkill fieldfare Turdus pilaris.Malaria journal · 2022Article
- First Report of TTSuV1 in Domestic Swiss Pigs.Viruses · 2022Article
- Active equine parvovirus-hepatitis infection is most frequently detected in Austrian horses of advanced age.Equine veterinary journal · 2022Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The value of next-generation sequencing (NGS)-based applications for testing purposes in human medicine is widely recognized. Although NGS-based metagenomic screening may be of interest in veterinary medicine, in particular for intensively farmed livestock species such as pigs, there is a lack of protocols tailored to veterinary requirements, likely because of the high diversity of species and samples. Therefore, we developed an NGS-based protocol for use in veterinary virology and present here different applications in porcine medicine. To develop the protocol, each step of sample preparation was optimized using porcine samples spiked with various RNA and DNA viruses. The resulting protocol was tested with clinical samples previously confirmed to be positive for specific viruses by a diagnostic laboratory. Additionally, we validated the protocol in an NGS viral metagenomics ring trial and tested the protocol on viral multiplex reference material (NIBSC, U.K.). We applied our ViroScreen protocol successfully for 1) virus identification, 2) virus characterization, and 3) herd screening. We identified torque teno sus virus and atypical porcine pestivirus in a neurologic case, determined the full-length genome sequence of swine influenza A virus in field samples, and screened pigs using pen floor fecal samples and chewing rope liquid.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.