ArticleAnnals of the New York Academy of Sciences2021
Erythrocyte transketolase activity coefficient (ETKAC) assay protocol for the assessment of thiamine status.
Article in Annals of the New York Academy of Sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06223360 (A Seamless Phase 2A-Phase 2B Randomized Double-Blind Placebo- Controlled Trial to Evaluate the Safety and Efficacy of Benfotiamine in Patients With Early Alzheimer's Disease), which is not on this map. Cited by 31 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
A Seamless Phase 2A-Phase 2B Randomized Double-Blind Placebo- Controlled Trial to Evaluate the Safety and Efficacy of Benfotiamine in Patients With Early Alzheimer's Disease (BenfoTeam)
Who cites it
31 citing papers in PubMed, 54 citations in OpenAlex.
- Impact of thiamin supplementation on thiamin pyrophosphate effect and cardiac function in pediatric heart disease patients on diuretics: a randomized controlled trial.Scientific reports · 2026Trial
- Low-dose thiamine supplementation of lactating Cambodian mothers improves human milk thiamine concentrations: a randomized controlled trial.The American journal of clinical nutrition · 2021Trial
- Functional thiamine deficiency in patients with chronic kidney disease in Northeast Thailand.Future science OA · 2026Article
- Reassessing Transketolase Assays: Methodological Considerations for Detecting Functional Thiamine Deficiency.Annals of the New York Academy of Sciences · 2026Review
- Thiamine Allocation and Deficiency Status Throughout the Life Cycle of Cod.Ecology and evolution · 2026Article
- Article
- Scoping review of micronutrient imbalances, clinical manifestations, and interventions.World journal of methodology · 2025Review
- Inadequate Status of Multiple B Vitamins is Common Among Women in Oromia Region, Ethiopia.Current developments in nutrition · 2025Article
- Micronutrient Biomarker Selection and Assay Methods and Performance in Double-Blind, Randomized, Controlled Micronutrient Dose Response (MiNDR) Trials among Women of Reproductive Age and Pregnant Women in Rural Bangladesh.Current developments in nutrition · 2025Article
- Factors associated with thiamin deficiency in pediatric patients with heart disease and receiving diuretics: a single-center study.Clinical and experimental pediatrics · 2025Article
- Thiamine Concentration in Human Milk Is Correlated With Maternal and Infant Thiamine Status: A Cross-Sectional Analysis of the Lao Thiamine Study.Maternal & child nutrition · 2025Observational
- Thiamine deficiency in US veterans with obesity.Obesity (Silver Spring, Md.) · 2025Observational
- The Impact of Delayed Processing of Chilled Whole Blood Specimens on the Measurement of Nutritional Biomarkers in the United Kingdom National Diet and Nutrition Survey Rolling Programme.The Journal of nutrition · 2024Article
- Assessment of Erythrocyte Transketolase, Whole Blood Thiamine Diphosphate, and Human Milk Thiamine Concentrations to Identify Infants and Young Children Responding Favorably to Therapeutic Thiamine Administration: Findings from the Lao Thiamine Study, a Prospective Cohort Study.Current developments in nutrition · 2024Article
- A Predictive Model for Thiamine Responsive Disorders Among Infants and Young Children: Results from a Prospective Cohort Study in Lao People's Democratic Republic.The Journal of pediatrics · 2024Article
- Nystagmus in a child with nephrotic syndrome.BMJ case reports · 2024Article
- Risk factors for anaemia among women and their young children hospitalised with suspected thiamine deficiency in northern Lao PDR.Maternal & child nutrition · 2024Article
- Article
- Article
- Correlation of Fasting Blood Sugar and Glycated Hemoglobin (HbA1c) With Thiamine Levels in Diabetic Patients.Cureus · 2023Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vitamin B1 (thiamine) is an essential nutrient that acts as a cofactor for a number of metabolic processes, particularly in energy metabolism. Symptoms of classic thiamine deficiency are recognized as beriberi, although clinical symptoms are nonspecific and recognition of subclinical deficiency is difficult. Therefore, reliable biomarkers of thiamine status are required. Thiamine diphosphate is a cofactor for transketolase, including erythrocyte transketolase (ETK). The ETK activity assay as an indirect, functional marker of thiamine status has been used for over 50 years. The ETK activity assay provides a sensitive and specific biomarker of thiamine status; however, there is a lack of consensus over the cutoffs for deficiency, partly due to a lack of assay harmonization. Here, we provide a step-by-step protocol for the measurement of ETK activity and the calculation of the ETK activity coefficient, including detailed explanations of equipment and chemicals required and guidance for quality control procedures. Harmonization of the protocol will provide the basis for the development of internationally recognized cutoffs for thiamine insufficiency. The establishment of quality control materials and a quality assurance scheme are recommended to provide reliability. This will ensure that the ETK activity assay remains an important method for the assessment of thiamine status.
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