ArticleMicrobial cell factories2020
Maximizing PHB content in Synechocystis sp. PCC 6803: a new metabolic engineering strategy based on the regulator PirC.
Article in Microbial cell factories, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Engineering primary metabolism for sustainable production of isoprene in Synechocystis sp. PCC 6803.Microbial cell factories · 2026Article
- Conversion of COMicrobial cell factories · 2026Article
- From pond to platform: howJournal of bacteriology · 2026Review
- Natural Deep Eutectic Solvents for PHB Recovery: Mechanistic Insights and Implications for Sustainable Downstream Processing.Polymers · 2026Review
- Cultivation in a Natural Microbial Community Enhances the Industrial Performance of a Genetically Engineered Cyanobacterium for Bioplastic Production.Microbial biotechnology · 2026Article
- Enhanced sucrose production by controlling carbon flux through CfrA expression in Synechocystis sp. PCC 6803.Microbial cell factories · 2025Article
- Controlled Carbon Loss: Threshold-Dependent Overflow Metabolism inMicroorganisms · 2025Article
- The toxic effects of meta-tyrosine are related to its misincorporation into the proteome and to altered metabolism in cyanobacteria.The FEBS journal · 2025Article
- Article
- Greening the Production of Indigo Blue Exploiting Light and a Recombinant Synechocystis sp. PCC6803 Strain Expressing the Enzyme mFMO.Microbial biotechnology · 2025Article
- A novel recombinant PHB production platform in filamentous cyanobacteria avoiding nitrogen starvation while preserving cell viability.Microbial cell factories · 2025Article
- Autotrophic bacterial production of polyhydroxyalkanoates using carbon dioxide as a sustainable carbon source.Frontiers in bioengineering and biotechnology · 2025Review
- Triple-Gene Overexpression of the AcrA-AcrB-TolC Transporter System inInternational journal of molecular sciences · 2024Article
- A Comparison of the Effects of Continuous Illumination and Day/Night Regimes on PHB Accumulation inLife (Basel, Switzerland) · 2024Article
- Deciphering the genetic landscape of enhanced poly-3-hydroxybutyrate production in Synechocystis sp. B12.Biotechnology for biofuels and bioproducts · 2024Article
- Double blocking of carbon metabolism causes a large increase of Calvin-Benson cycle compounds in cyanobacteria.Plant physiology · 2024Article
- Natural Polyhydroxyalkanoates-An Overview of Bacterial Production Methods.Molecules (Basel, Switzerland) · 2024Review
- Extracellular Poly(hydroxybutyrate) Bioplastic Production Using Surface Display Techniques.ACS materials Au · 2024Article
- The primary carbon metabolism in cyanobacteria and its regulation.Frontiers in plant science · 2024Review
- Development ofFrontiers in microbiology · 2024Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundPHB (poly-hydroxy-butyrate) represents a promising bioplastic alternative with good biodegradation properties. Furthermore, PHB can be produced in a completely carbon-neutral fashion in the natural producer cyanobacterium Synechocystis sp. PCC 6803. This strain has been used as model system in past attempts to boost the intracellular production of PHB above ~ 15% per cell-dry-weight (CDW).
resultsWe have created a new strain that lacks the regulatory protein PirC (product of sll0944), which exhibits a higher activity of the phosphoglycerate mutase resulting in increased PHB pools under nutrient limiting conditions. To further improve the intracellular PHB content, two genes involved in PHB metabolism, phaA and phaB, from the known producer strain Cupriavidus necator, were introduced under the control of the strong promotor PpsbA2. The resulting strain, termed PPT1 (ΔpirC-REphaAB), produced high amounts of PHB under continuous light as well under a day-night regime. When grown in nitrogen and phosphorus depleted medium, the cells produced up to 63% per CDW. Upon the addition of acetate, the content was further increased to 81% per CDW. The produced polymer consists of pure PHB, which is highly isotactic.
conclusionThe amounts of PHB achieved with PPT1 are the highest ever reported in any known cyanobacterium and demonstrate the potential of cyanobacteria for a sustainable, industrial production of PHB.
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