Evidence map›Paper›PMID 33352414›Full record

ArticleUltramicroscopy2021

Robust scan synchronized force-fluorescence imaging.

Patrick Schmidt, John Lajoie, Sanjeevi Sivasankar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ultramicroscopy, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Patrick SchmidtDepartment of Biomedical Engineering, University of California, Davis, CA, 95616, USA; Department of Electrical and Computer Engineering, Iowa State University, Ames, IA, 50011, USA.
John LajoieDepartment of Physics and Astronomy, Iowa State University, Ames, IA, 50011, USA.
Sanjeevi SivasankarDepartment of Biomedical Engineering, University of California, Davis, CA, 95616, USA. Electronic address: ssivasankar@ucdavis.edu.

Funding

Microscope for ultrasensitive measurement of single-molecule interaction and conformationR01GM121885 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SIVASANKAR, SANJEEVI · 2017 to 2024
$2.5M
NIGMS NIH HHS R01 GM121885
6 · The paper itself

Abstract

Simultaneous atomic force microscope (AFM) and sample scanning confocal fluorescence microscope measurements are widely used to obtain mechanistic and structural insights into protein dynamics in live cells. However, the absence of a robust technique to synchronously scan both AFM and confocal microscope piezo stages makes it difficult to visualize force-induced changes in fluorescent protein distribution in cells.  To address this challenge, we have built an integrated AFM-confocal fluorescence microscope platform that implements a synchronous scanning method which eliminates image artifacts from piezo motion ramping, produces accurate pixel binning and enables the collection of a scanned image of a sample while applying force to a single point on the sample. As proof of principle, we use this instrument to monitor the redistribution of fluorescent E-cadherin, an essential transmembrane protein, in live cells, upon application of mechanical force.

Indexed as

AnimalsCadherinsDogsMadin Darby Canine Kidney CellsMicroscopy, Atomic ForceMicroscopy, ConfocalMicroscopy, FluorescenceOptical ImagingCadherinsAFM-sample scanning confocal microscopeIntegrated AFM–fluorescence microscopePoint scanningSimultaneous force-fluorescence measurementsSynchronized scanning

Identifiers

PMID33352414
PMCPMC8761069

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.