ArticlePLoS biology2020
Single-cell transcriptome landscape of ovarian cells during primordial follicle assembly in mice.
Article in PLoS biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers, 1 of them a synthesis that pooled it.
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Who cites it
83 citing papers in PubMed, 1 synthesis or guideline pooled it, 145 citations in OpenAlex.
- Modulation of Female Reproductive Functions through Nerve Growth Factor: A Systematic Review.Expert reviews in molecular medicine · 2026Pooled it
- Characterization of Ovarian Cell Types and Molecular Signatures in Mauremys reevesii Using Single-Cell Sequencing.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- In vivo labeling-based proteomic analysis of early follicle oocytes and cisplatin-induced alterations in mice.Nature communications · 2026Article
- Developmental and functional landscape of theca cells in ovarian biology.Biological research · 2026Review
- Dissecting the transcriptomic signature of porcine oocyte during primordial follicle formation from single cell resolution.Journal of ovarian research · 2026Article
- Gene regulatory networks orchestrating oocyte fate bifurcation in primordial follicles revealed by single-cell transcriptomics.Communications biology · 2026Article
- Cross-species single-cell transcriptomic analyses reveal evolutionary conservation and diversification of ovarian tissues.Journal of animal science and biotechnology · 2026Article
- Coordinating meiotic prophase I progression and early oocyte differentiation.Development (Cambridge, England) · 2026Article
- Transcriptomic Analysis of Fermented Chinese Chive Selectively Attenuating Deoxynivalenol-Induced Ovarian Toxicity in Mice.Antioxidants (Basel, Switzerland) · 2026Article
- USP9X as a Candidate Mediator of Prenatal Aspirin-Induced Ovarian Reserve Reduction in Offspring Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Single-cell sequencing and organoids: applications in organ development and disease.Molecular biomedicine · 2025Review
- Article
- Unveiling the potential targets and mechanisms of DEHP/BPA exposure on PCOS: insights from network toxicology and molecular docking.Journal of ovarian research · 2025Article
- CCAAT/enhancer binding protein beta (C/EBPβ) regulates the formation of the ovarian reserve.Communications biology · 2025Article
- Article
- Article
- Spatiotemporal and single-cell atlases to dissect regional specific cell types of the developing ovary.Communications biology · 2025Article
- Inhibition of ferroptosis counteracts the advanced maternal age-induced oocyte deterioration.Cell death and differentiation · 2025Article
- A multi-omic single-cell landscape of the aging mouse ovary.GeroScience · 2025Article
- Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Primordial follicle assembly in the mouse occurs during perinatal ages and largely determines the ovarian reserve that will be available to support the reproductive life span. The development of primordial follicles is controlled by a complex network of interactions between oocytes and ovarian somatic cells that remain poorly understood. In the present research, using single-cell RNA sequencing performed over a time series on murine ovaries, coupled with several bioinformatics analyses, the complete dynamic genetic programs of germ and granulosa cells from E16.5 to postnatal day (PD) 3 were reported. Along with confirming the previously reported expression of genes by germ cells and granulosa cells, our analyses identified 5 distinct cell clusters associated with germ cells and 6 with granulosa cells. Consequently, several new genes expressed at significant levels at each investigated stage were assigned. By building single-cell pseudotemporal trajectories, 3 states and 1 branch point of fate transition for the germ cells were revealed, as well as for the granulosa cells. Moreover, Gene Ontology (GO) term enrichment enabled identification of the biological process most represented in germ cells and granulosa cells or common to both cell types at each specific stage, and the interactions of germ cells and granulosa cells basing on known and novel pathway were presented. Finally, by using single-cell regulatory network inference and clustering (SCENIC) algorithm, we were able to establish a network of regulons that can be postulated as likely candidates for sustaining germ cell-specific transcription programs throughout the period of investigation. Above all, this study provides the whole transcriptome landscape of ovarian cells and unearths new insights during primordial follicle assembly in mice.
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