ArticleThe Journal of cell biology2021
A selective transmembrane recognition mechanism by a membrane-anchored ubiquitin ligase adaptor.
Article in The Journal of cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Vsb1, Ypq1, and Ypq2 control dynamic cationic amino acid storage in the yeast vacuole.Life science alliance · 2026Article
- Ubiquitination of secretory granules promotes their crinophagic degradation in Drosophila.FEBS letters · 2026Article
- Membrane-embedded polar residues target membrane proteins for degradation by the quality control protease FtsH.Nature communications · 2026Article
- The role of fungal G protein-coupled receptors in interspecies cell-cell communication.FEMS microbiology reviews · 2026Review
- JIP4 deficiency causes a lysosomal storage disease arising from impaired cystine efflux.bioRxiv : the preprint server for biology · 2025Article
- Lysosomal Repair in Health and Disease.Journal of cellular physiology · 2025Review
- Protocol for purifying and reconstituting a vacuole membrane transporter Ypq1 into proteoliposomes.STAR protocols · 2024Article
- Lysosomal membrane transporter purification and reconstitution for functional studies.Molecular biology of the cell · 2024Article
- GCAF(TMEM251) regulates lysosome biogenesis by activating the mannose-6-phosphate pathway.Nature communications · 2022Article
- Built to last: lysosome remodeling and repair in health and disease.Trends in cell biology · 2022Review
- An optimized protocol to analyze membrane protein degradation in yeast using quantitative western blot and flow cytometry.STAR protocols · 2022Article
- ESCRT, not intralumenal fragments, sorts ubiquitinated vacuole membrane proteins for degradation.The Journal of cell biology · 2021Article
- A conserved ubiquitin- and ESCRT-dependent pathway internalizes human lysosomal membrane proteins for degradation.PLoS biology · 2021Article
- The Ubiquitin Switch in Plant Stress Response.Plants (Basel, Switzerland) · 2021Review
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5 authors.
Funding
Abstract
While it is well-known that E3 ubiquitin ligases can selectively ubiquitinate membrane proteins in response to specific environmental cues, the underlying mechanisms for the selectivity are poorly understood. In particular, the role of transmembrane regions, if any, in target recognition remains an open question. Here, we describe how Ssh4, a yeast E3 ligase adaptor, recognizes the PQ-loop lysine transporter Ypq1 only after lysine starvation. We show evidence of an interaction between two transmembrane helices of Ypq1 (TM5 and TM7) and the single transmembrane helix of Ssh4. This interaction is regulated by the conserved PQ motif. Strikingly, recent structural studies of the PQ-loop family have suggested that TM5 and TM7 undergo major conformational changes during substrate transport, implying that transport-associated conformational changes may determine the selectivity. These findings thus provide critical information concerning the regulatory mechanism through which transmembrane domains can be specifically recognized in response to changing environmental conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.