Evidence map›Paper›PMID 33350092›Full record

ArticleJournal of cellular and molecular medicine2021

Exosomes from microRNA-126 overexpressing mesenchymal stem cells promote angiogenesis by targeting the PIK3R2-mediated PI3K/Akt signalling pathway.

Lei Zhang, Pengrong Ouyang, Gaole He, Xiaowei Wang, Defu Song, Yijun Yang, Xijing He

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
79citing papers in PubMed, 4 pooled it
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

79 citing papers in PubMed, 4 syntheses or guidelines pooled it, 98 citations in OpenAlex.

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  14. Crosstalk of extracellular vesicles in maternal-fetal interaction.Frontiers in cell and developmental biology · 2026
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19 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Lei ZhangDepartment of Orthopaedic Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.ORCID 0000-0003-0671-0110
Pengrong OuyangDepartment of Orthopaedic Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Gaole HeDepartment of Spine Surgery, Hong Hui Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Xiaowei WangDepartment of Orthopaedic Surgery, Xi'an Children's Hospital, Xi'an, Shaanxi Province, China.
Defu SongDepartment of Orthopaedic Surgery, Xi'an Children's Hospital, Xi'an, Shaanxi Province, China.
Yijun YangDepartment of Orthopaedic Surgery, Xi'an Children's Hospital, Xi'an, Shaanxi Province, China.
Xijing HeDepartment of Orthopaedic Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Province, China.
Xi’an Children’s Hospital · CNSecond Affiliated Hospital of Xi'an Jiaotong University · CNXi'an Honghui Hospital · CNXi’an International University · CN

Funding

the National Key Research and Development Project 2018YFE0114200The National Natural Science Foundation of China 81771349
6 · The paper itself

Abstract

microRNA-126 (miR-126), an endothelial-specific miRNA, is associated with vascular homeostasis and angiogenesis. However, the efficiency of miR-126-based treatment is partially compromised due to the low efficiency of miRNA delivery in vivo. Lately, exosomes have emerged as a natural tool for therapeutic molecule delivery. Herein, we investigated whether exosomes derived from bone marrow mesenchymal stem cells (BMMSCs) can be utilized to deliver miR-126 to promote angiogenesis. Exosomes were isolated from BMMSCs overexpressed with miR-126 (Exo-miR-126) by ultracentrifugation. In vitro study, Exo-miR-126 treatment promoted the proliferation, migration and angiogenesis of human umbilical vein endothelial cells (HUVECs). Furthermore, the gene/protein expression of angiogenesis-related vascular endothelial growth factor (VEGF) and angiotensin-1 (Ang-1) were up-regulated after incubation with Exo-miR-126. Additionally, the expression level of phosphoinositol-3 kinase regulatory subunit 2 (PIK3R2) showed an inverse correlation with miR-126 in HUVECs. Particularly, the Exo-miR-126 treatment contributed to enhanced angiogenesis of HUVECs by targeting PIK3R2 to activate the PI3K/Akt signalling pathway. Similarly, Exo-miR-126 administration profoundly increased the number of newly formed capillaries in wound sites and accelerated the wound healing in vivo. The results demonstrate that exosomes derived from BMMSCs combined with miR-126 may be a promising strategy to promote angiogenesis.

Indexed as

Signal TransductionCell MovementCell ProliferationExosomesHumansHuman Umbilical Vein Endothelial CellsMesenchymal Stem CellsMicroRNAsNeovascularization, PhysiologicPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktWound HealingMicroRNAsMIRN126 microRNA, humanPhosphatidylinositol 3-Kinasesphosphoinositol-3 kinase regulatory subunit 2, humanProto-Oncogene Proteins c-aktangiogenesisbone marrow mesenchymal stem cellsexosomesmicroRNA-126

Identifiers

PMID33350092
PMCPMC7882955
OpenAlexW3116986630

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.