Evidence map›Paper›PMID 33349704›Full record

ArticleNature protocols2021

Labeling and tracking of immune cells in ex vivo human skin.

Feline E Dijkgraaf, Mireille Toebes, Mark Hoogenboezem, Marjolijn Mertz, David W Vredevoogd, Tiago R Matos, Marcel B M Teunissen, Rosalie M Luiten, Ton N Schumacher

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PubMed Publisher
In one paragraph

Article in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 50% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. The Development of Human Ex Vivo Models of Inflammatory Skin Conditions.International journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Feline E DijkgraafDivision of Molecular Oncology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
Mireille ToebesDivision of Molecular Oncology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
Mark HoogenboezemResearch Facility, Sanquin Amsterdam, Amsterdam, the Netherlands.
Marjolijn MertzBioImaging Facility, The Netherlands Cancer Institute, Amsterdam, the Netherlands.ORCID http://orcid.org/0000-0002-9188-3579
David W VredevoogdDivision of Molecular Oncology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, the Netherlands.
Tiago R MatosDepartment of Dermatology and Netherlands Institute for Pigment Disorders, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands.ORCID http://orcid.org/0000-0003-2864-8207
Marcel B M TeunissenDepartment of Dermatology and Netherlands Institute for Pigment Disorders, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands.ORCID http://orcid.org/0000-0003-1119-6662
Rosalie M LuitenDepartment of Dermatology and Netherlands Institute for Pigment Disorders, Amsterdam University Medical Centers, University of Amsterdam, Amsterdam, the Netherlands.
Ton N SchumacherDivision of Molecular Oncology and Immunology, Oncode Institute, The Netherlands Cancer Institute, Amsterdam, the Netherlands. t.schumacher@nki.nl.ORCID http://orcid.org/0000-0003-0517-8804
The Netherlands Cancer Institute · NLAmsterdam University Medical Centers · NLSanquin · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human skin harbors various immune cells that are crucial for the control of injury and infection. However, the current understanding of immune cell function within viable human skin tissue is limited. We developed an ex vivo imaging approach in which fresh skin biopsies are mounted and then labeled with nanobodies or antibodies against cell surface markers on tissue-resident memory CD8

Indexed as

AnimalsBiopsyCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCell Culture TechniquesHumansMiceSkinStaining and Labeling

Identifiers

PMID33349704
OpenAlexW3114288987

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.