ArticleNature protocols2021
Design, assembly, and characterization of membrane-spanning DNA nanopores.
Article in Nature protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 63 citations in OpenAlex.
- Morphology-coupled formation and reversible gating of membrane channels in synthetic cells using reconfigurable DNA nanorafts.Nature protocols · 2026Review
- Membrane-Spanning Nanopores Formed from Nucleic Acids.Chemical reviews · 2026Review
- Intracellular neuronal recordings across DNA tiles.Nature nanotechnology · 2026Article
- Nanopipette-DNA-Nanopore Hybrid System for Small Molecule Detection.ChemPlusChem · 2026Article
- Review
- A lumen-tunable triangular DNA nanopore for molecular sensing and cross-membrane transport.Nature communications · 2024Article
- DNA-empowered synthetic cells as minimalistic life forms.Nature reviews. Chemistry · 2024Review
- Nanopore DNA sequencing technologies and their applications towards single-molecule proteomics.Nature chemistry · 2024Review
- Precise construction of DNA origami-based materials for functional regulation on biological interface.Smart molecules : open access · 2024Review
- Rationally Designed DNA-Based Scaffolds and Switching Probes for Protein Sensing.Advances in biochemical engineering/biotechnology · 2024Review
- Net-Shaped DNA Nanostructures Designed for Rapid/Sensitive Detection and Potential Inhibition of the SARS-CoV-2 Virus.Journal of the American Chemical Society · 2023Article
- Functionalization and higher-order organization of liposomes with DNA nanostructures.Nature communications · 2023Article
- Structure and dynamics of an archetypal DNA nanoarchitecture revealed via cryo-EM and molecular dynamics simulations.Nature communications · 2023Article
- Creating complex protocells and prototissues using simple DNA building blocks.Nature communications · 2023Article
- Ethane groups modified DNA nanopores to prolong the dwell time on live cell membranes for transmembrane transport.Frontiers in chemistry · 2023Article
- Network topology-directed design of molecular CPU for cell-like dynamic information processing.Science advances · 2022Article
- Digging into the biophysical features of cell membranes with lipid-DNA conjugates.Quarterly reviews of biophysics · 2022Review
- A reversibly gated protein-transporting membrane channel made of DNA.Nature communications · 2022Article
- Triggered Assembly of a DNA-Based Membrane Channel.Journal of the American Chemical Society · 2022Article
- Designer DNA nanostructures for viral inhibition.Nature protocols · 2022Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
DNA nanopores are bio-inspired nanostructures that control molecular transport across lipid bilayer membranes. Researchers can readily engineer the structure and function of DNA nanopores to synergistically combine the strengths of DNA nanotechnology and nanopores. The pores can be harnessed in a wide range of areas, including biosensing, single-molecule chemistry, and single-molecule biophysics, as well as in cell biology and synthetic biology. Here, we provide a protocol for the rational design of nanobarrel-like DNA pores and larger DNA origami nanopores for targeted applications. We discuss strategies for the pores' chemical modification with lipid anchors to enable them to be inserted into membranes such as small unilamellar vesicles (SUVs) and planar lipid bilayers. The procedure covers the self-assembly of DNA nanopores via thermal annealing, their characterization using gel electrophoresis, purification, and direct visualization with transmission electron microscopy and atomic force microscopy. We also describe a gel assay to determine pore-membrane binding and discuss how to use single-channel current recordings and dye flux assays to confirm transport through the pores. We expect this protocol to take approximately 1 week to complete for DNA nanobarrel pores and 2-3 weeks for DNA origami pores.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.