ArticlePloS one2020
Whole genome sequencing of Clostridioides difficile PCR ribotype 046 suggests transmission between pigs and humans.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 27 citations in OpenAlex.
- Changes in the Epidemiology of Clostridioides difficile Infection.Infectious disease clinics of North America · 2025Review
- The Effect of Climate Change on Emergence and Evolution of Zoonotic Diseases in Asia.Zoonoses and public health · 2025Review
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- Evaluation of nationwide analysis surveillance for methicillin-resistantMicrobial genomics · 2025Article
- High genetic similarity betweenFrontiers in public health · 2025Article
- Genomic epidemiology ofEmerging microbes & infections · 2024Article
- Assessing the Risk of SpreadingAnimals : an open access journal from MDPI · 2024Article
- Fast and Economic Microarray-Based Detection of Species-, Resistance-, and Virulence-Associated Genes in Clinical Strains of Vancomycin-Resistant Enterococci (VRE).Sensors (Basel, Switzerland) · 2024Article
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- The inhibitory effects of live and UV-killed Akkermansia muciniphila and its derivatives on cytotoxicity and inflammatory response induced by Clostridioides difficile RT001 in vitro.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Ribotypes and New Virulent Strains Across Europe.Advances in experimental medicine and biology · 2024Review
- New Multilocus Sequence Typing Scheme for Enterococcus faecium Based on Whole Genome Sequencing Data.Microbiology spectrum · 2023Article
- Update on Commonly Used Molecular Typing Methods forMicroorganisms · 2023Review
- Genetic and phenotypic characteristics of Clostridium (Clostridioides) difficile from canine, bovine, and pediatric populations.Anaerobe · 2022Article
- Comparison of Whole-Genome Sequence-Based Methods and PCR Ribotyping for Subtyping of Clostridioides difficile.Journal of clinical microbiology · 2022Article
- Assessment of the Transmission Dynamics ofFrontiers in microbiology · 2022Article
- Antimicrobial resistance in Clostridioides difficile.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Review
- Comparative genomic and transmission analysis ofEmerging microbes & infections · 2021Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA zoonotic association has been suggested for several PCR ribotypes (RTs) of Clostridioides difficile. In central parts of Sweden, RT046 was found dominant in neonatal pigs at the same time as a RT046 hospital C. difficile infection (CDI) outbreak occurred in the southern parts of the country.
objectiveTo detect possible transmission of RT046 between pig farms and human CDI cases in Sweden and investigate the diversity of RT046 in the pig population using whole genome sequencing (WGS).
methodsWGS was performed on 47 C. difficile isolates from pigs (n = 22), the farm environment (n = 7) and human cases of CDI (n = 18). Two different core genome multilocus sequencing typing (cgMLST) schemes were used together with a single nucleotide polymorphisms (SNP) analysis and the results were related to time and location of isolation of the isolates.
resultsThe pig isolates were closely related (≤6 cgMLST alleles differing in both cgMLST schemes) and conserved over time and were clearly separated from isolates from the human hospital outbreak (≥76 and ≥90 cgMLST alleles differing in the two cgMLST schemes). However, two human isolates were closely related to the pig isolates, suggesting possible transmission. The SNP analysis was not more discriminate than cgMLST.
conclusionNo general pattern suggesting zoonotic transmission was apparent between pigs and humans, although contrasting results from two isolates still make transmission possible. Our results support the need for high resolution WGS typing when investigating hospital and environmental transmission of C. difficile.
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