ArticleFEBS open bio2021
Platelet-derived extracellular vesicles are increased in sera of Alzheimer's disease patients, as revealed by Tim4-based assays.
Article in FEBS open bio, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- TEMIS: A temperature-controlled microwave irradiation system for enhancing extracellular vesicle secretion.Biochemistry and biophysics reports · 2026Article
- Review
- Quantitative Proteomic Analysis of APP/PS1 Transgenic Mice.Current Alzheimer research · 2026Article
- Platelet Extracellular Vesicles as Natural Delivery Vehicles for Mitochondrial Dysfunction Therapy?ACS biomaterials science & engineering · 2025Review
- One immune cell to bind them all: platelet contribution to neurodegenerative disease.Molecular neurodegeneration · 2024Review
- Single cell transcriptome analysis of the THY-Tau22 mouse model of Alzheimer's disease reveals sex-dependent dysregulations.Cell death discovery · 2024Article
- FTIR Spectroscopy and Blood-Derived Extracellular Vesicles Duo in Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2024Article
- Surface Glycan Profiling of Extracellular Vesicles by Lectin Microarray and Glycoengineering for Control of Cellular Interactions.Pharmaceutical research · 2023Article
- Extracellular vesicles released from ganglioside GD2-expressing melanoma cells enhance the malignant properties of GD2-negative melanomas.Scientific reports · 2023Article
- Platelet mitochondria, a potent immune mediator in neurological diseases.Frontiers in physiology · 2023Review
- Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence.NPJ Regenerative medicine · 2022Article
- CD63-positive extracellular vesicles are potential diagnostic biomarkers of pancreatic ductal adenocarcinoma.BMC gastroenterology · 2022Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alzheimer's disease (AD) is the most common form of dementia, characterized by the accumulation of β-amyloid plaques and the formation of neurofibrillary tangles. Extracellular vesicles (EVs) are small vesicles surrounded by a lipid bilayer membrane, which may be involved in the progression of AD. Glycans are essential building blocks of EVs, and we hypothesized that EV glycans may reflect pathological conditions of various diseases. Here, we performed glycan profiling of EVs prepared from sera of three AD patients (APs) compared to three healthy donors (HDs) using lectin microarray. Distinct glycan profiles were observed. Mannose-binding lectins exhibited significantly higher signals for AP-derived EVs than HD-derived EVs. Lectin blotting using mannose-binding lectin (rPALa) showed a single protein band at ~ 80 kDa exclusively in AP-derived EVs. LC-MS/MS analysis identified a protein band precipitated by rPALa as CD61, a marker of platelet-derived exosomes (P-Exo). Sandwich assays using Tim4 with specificity for phosphatidylserine on EVs and antibodies against P-Exo markers (CD61, CD41, CD63, and CD9) revealed that P-Exo is significantly elevated in sera of APs (n = 16) relative to age- and sex-matched HDs (n = 16). Tim4-αCD63 showed the highest value for the area under the curve (0.957) for discriminating APs from HDs, which should lead to a better understanding of AD pathology and may facilitate the development of a novel diagnostic method for AD.
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