Evidence map›Paper›PMID 33345421›Full record

ReviewCytometry. Part A : the journal of the International Society for Analytical Cytology2021

Comprehensive phenotyping of endothelial cells using flow cytometry 1: Murine.

Dillon Grant, Nicholas Wanner, Matthew Frimel, Serpil Erzurum, Kewal Asosingh

Open access · greenAbstract readReview
In one paragraph

Review in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.8field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Review
  6. Review
  7. Article
  8. Using the Autofluorescence Finder on the Sony ID7000Frontiers in bioengineering and biotechnology · 2022
    Article
  9. Comprehensive phenotyping of endothelial cells using flow cytometry 2: Human.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Dillon GrantDepartment of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA.
Nicholas WannerDepartment of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA.
Matthew FrimelDepartment of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA.
Serpil ErzurumDepartment of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA.
Kewal AsosinghDepartment of Inflammation and Immunity, Cleveland Clinic, Cleveland, Ohio, USA.
Cleveland Clinic · US

Funding

Targeting pathological hyaluronan matricesP01HL103453 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI ERZURUM, SERPIL C. · 2011 to 2020
$26.8M
NITRIC OXIDE PRODUCTION AND REACTIONS IN THE LUNGR01HL060917 · NHLBI · CLEVELAND CLINIC LERNER COM-CWRU · PI Serpil C. Erzurum · 1999 to 2026
$6.3M
NHLBI NIH HHS P01 HL103453NHLBI NIH HHS R01 HL060917
6 · The paper itself

Abstract

The endothelium forms a selective barrier between circulating blood or lymph and surrounding tissue. Endothelial cells play an essential role in vessel homeostasis, and identification of these cells is critical in vascular biology research. However, characteristics of endothelial cells differ depending on the location and type of blood or lymph vessel. Endothelial cell subsets are numerous and often identified using different flow cytometric markers, making immunophenotyping these cells complex. In part 1 of this two part review series, we present a comprehensive overview of markers for the flow cytometric identification and phenotyping of murine endothelial subsets. These subsets can be distinguished using a panel of cell surface and intracellular markers shared by all endothelial cells in combination with additional markers of specialized endothelial cell types. This review can be used to determine the best markers for identifying and phenotyping desired murine endothelial cell subsets.

Indexed as

Endothelial CellsAnimalsBiomarkersFlow CytometryImmunophenotypingMiceBiomarkersendothelial cellendothelial cell subsetflow cytometryimmunophenotypingmurinephenotyping

Identifiers

PMID33345421
PMCPMC8593928
OpenAlexW3115151384

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.