Evidence map›Paper›PMID 33344524›Full record

ArticleFrontiers in veterinary science2020

Quantitative Proteomic Analysis of Duck Embryo Fibroblasts Infected With Novel Duck Reovirus.

Yudong Yang, Lin Li, Xingpo Liu, Meijie Jiang, Jun Zhao, Xuesong Li, Cui Zhao, Hui Yi, Sidang Liu, Ning Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in veterinary science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.1field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 1 country.

Yudong YangShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Taian, China.
Lin LiTaian City Central Hospital, Taian, China.
Xingpo LiuShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Meijie JiangTaian City Central Hospital, Taian, China.
Jun ZhaoShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Taian, China.
Xuesong LiShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Cui ZhaoTaian City Animal Husbandry and Veterinary Service Center, Taian, China.
Hui YiTaian City Animal Husbandry and Veterinary Service Center, Taian, China.
Sidang LiuShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Taian, China.
Ning LiShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Taian, China.
Shandong Agricultural University · CNNational Animal Husbandry Service · CNShanghai Veterinary Research Institute · CNTaian City Central Hospital · CNChina Animal Disease Control Center · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The novel duck reovirus (NDRV) can cause hemorrhage and necrosis on the spleen of Pekin ducks; this disease has resulted in great economic losses to the duck industry. However, the molecular pathogenesis of NDRV remains poorly understood. In the current study, the quantitative proteomic analysis of NDRV-infected duck embryo fibroblasts was performed to explore the cellular protein changes in response to viral infection through iTRAQ coupled with the liquid chromatography (LC)-tandem mass spectrometry (MS/MS) method. A total of 6,137 proteins were obtained in cell samples at 24 h post-infection. Of these, 179 differentially expressed proteins (DEPs) were identified (cutoff set to 1.5-fold change), including 89 upregulated and 90 downregulated proteins. Bioinformatics analysis showed that DEPs can be divided into the cellular component, molecular function, and biological process; they were mainly involved in signal transduction, infectious diseases, cell growth and death, and the immune system. The subcellular localization of most proteins was in the cytoplasm. Importantly, the expressions of signal transducer and activator of transcription 1 (STAT1) and various interferon-stimulated genes (ISGs) were upregulated after NDRV infection. The mRNA transcripts of some ISGs were consistent with proteomic data, showing an increased trend. Results of our study suggested that NDRV infection can elicit strong expression changes of cellular proteins and activate the expression of ISGs from the point of quantitative proteomic analysis. The study provides a new insight into the understanding of NDRV pathogenesis.

Indexed as

cellular responseinterferon-stimulated genesiTRAQnovel duck reovirusproteomic analysis

Identifiers

PMID33344524
PMCPMC7738351
OpenAlexW3109992239

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.