Evidence map›Paper›PMID 33343059›Full record

ArticleJournal of magnetism and magnetic materials2021

Effects of fixatives on histomagnetic evaluation of iron in rodent spleen.

Kevin J Walsh, Stavan V Shah, Ping Wei, Samuel D Oberdick, Nicole M Karn, Dana M McTigue, Gunjan Agarwal

Open access · greenAbstract read
In one paragraph

Article in Journal of magnetism and magnetic materials, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Multimodal microscopic and spectroscopic characterisation of heterogeneous iron oxyhydroxides in the human globus pallidus.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026
    Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Kevin J WalshBiophysics Program (The Ohio State University).
Stavan V ShahDepartment of Biomedical Engineering (The Ohio State University).
Ping WeiDepartment of Neuroscience (The Ohio State University).
Samuel D OberdickPhysics Department (University of Colorado, Boulder).
Nicole M KarnDepartment of Chemistry and Biochemistry (The Ohio State University).
Dana M McTigueDepartment of Neuroscience (The Ohio State University).
Gunjan AgarwalBiophysics Program (The Ohio State University).
The Ohio State University · USApplied BioPhysics (United States) · USUniversity of Colorado Boulder · US

Funding

Molecular Biophysics predoctoral training at The Ohio State UniversityT32GM118291 · NIGMS · OHIO STATE UNIVERSITY · PI BUNDSCHUH, RALF A, KURET, JEFF · 2017 to 2021
$720k
NIGMS NIH HHS T32 GM118291
6 · The paper itself

Abstract

Characterizing the iron distribution in tissue sections is important for several pathologies. Iron content in excised tissue is typically analyzed via histochemical stains, which are dependent on sample preparation and staining protocols. In our recent studies, we examined how magnetic properties of iron can also be exploited to characterize iron distribution in tissue sections in a label free manner. To enable a histomagnetic characterization of iron in a wide variety of available tissues, it is important to extend it to samples routinely prepared for histochemical staining, which often involve use of chemical fixatives. In this study, we took a systematic approach to determine differences between unfixed and formalin-fixed murine spleen tissues in histomagnetic characterization of iron. Superconducting quantum interference device (SQUID) magnetometry and magnetic force microscopy (MFM) were used for macro- and micro-scale histomagnetic characterization. Perl's stain was used for histochemical characterization of ferric (Fe

Indexed as

ferritinhistologyironmagnetic force microscopymagnetometry

Identifiers

PMID33343059
PMCPMC7748249
OpenAlexW3106095677

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.