ArticleBiotechnology for biofuels2020
Homologous production, one-step purification, and proof of Na
Article in Biotechnology for biofuels, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- A characterized promoter suite enables metabolic rewiring of the acetogen Acetobacterium woodii toward ethanol production.Biotechnology for biofuels and bioproducts · 2026Article
- Rational engineering of facultative anaerobiosis enables commensal survival in the oxygenated gut.bioRxiv : the preprint server for biology · 2026Article
- Molecular principles of redox-coupled sodium pumping of the ancient Rnf machinery.Nature communications · 2025Article
- Harnessing acetogenic bacteria for one-carbon valorization toward sustainable chemical production.RSC chemical biology · 2024Review
- Sustainable metabolic engineering requires a perfect trifecta.Current opinion in biotechnology · 2023Review
- The oxidoreductase activity of Rnf balances redox cofactors during fermentation of glucose to propionate in Prevotella.Scientific reports · 2023Article
- Isolation, Genomic Sequence and Physiological Characterization ofApplied and environmental microbiology · 2023Article
- Mechanisms for Generating Low Potential Electrons across the Metabolic Diversity of Nitrogen-Fixing Bacteria.Applied and environmental microbiology · 2023Review
- Molecular Basis of the Electron Bifurcation Mechanism in the [FeFe]-Hydrogenase Complex HydABC.Journal of the American Chemical Society · 2023Article
- Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways.Biotechnology for biofuels and bioproducts · 2023Article
- Purification and structural characterization of the NaNature communications · 2022Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCapture and storage of the energy carrier hydrogen as well as of the greenhouse gas carbon dioxide are two major problems that mankind faces currently. Chemical catalysts have been developed, but only recently a group of anaerobic bacteria that convert hydrogen and carbon dioxide to acetate, formate, or biofuels such as ethanol has come into focus, the acetogenic bacteria. These biocatalysts produce the liquid organic hydrogen carrier formic acid from H
resultsWe have produced a His-tagged variant of the ferredoxin:NAD oxidoreductase (Rnf complex) from the model acetogen Acetobacterium woodii, solubilized the enzyme from the cytoplasmic membrane, and purified it by Ni DISCUSSION: We present a protocol for homologous production and purification for an Rnf complex. The enzyme catalyzed electron-transfer driven Na
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.