Evidence map›Paper›PMID 33342281›Full record

ArticleExperimental biology and medicine (Maywood, N.J.)2021

Conformation and protein interactions of intramolecular DNA and phosphorothioate four-way junctions.

Maria Troisi, Mitchell Klein, Andrew C Smith, Gaston Moorhead, Yonatan Kebede, Raymond Huang, Elliott Parker, Hector Herrada, Elizabeth Wade, Samara Smith and 4 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental biology and medicine (Maywood, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 1 institution in 1 country.

Maria TroisiDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Mitchell KleinDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Andrew C SmithDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Gaston MoorheadDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Yonatan KebedeDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Raymond HuangDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Elliott ParkerDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Hector HerradaDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Elizabeth WadeDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Samara SmithDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Payson BroomeDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Jonah HalsellDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Louis EstevezDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.
Anthony J BellDepartment of Chemistry and Biochemistry, University of San Diego, San Diego, CA 92110, USA.ORCID 0000-0003-3813-9043
University of San Diego · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The objectives of this study are to evaluate the structure and protein recognition features of branched DNA four-way junctions in an effort to explore the therapeutic potential of these molecules. The classic immobile DNA 4WJ, J1, is used as a matrix to design novel intramolecular junctions including natural and phosphorothioate bonds. Here we have inserted H2-type mini-hairpins into the helical termini of the arms of J1 to generate four novel intramolecular four-way junctions. Hairpins are inserted to reduce end fraying and effectively eliminate potential nuclease binding sites. We compare the structure and protein recognition features of J1 with four intramolecular four-way junctions:

Indexed as

Nucleic Acid ConformationAnimalsCircular DichroismDNAEndonucleasesHMGB1 ProteinNucleic Acid DenaturationPhosphorothioate OligonucleotidesProtein BindingProteinsRatsTemperatureDNAEndonucleasesHMGB1 ProteinPhosphorothioate OligonucleotidesProteinsDNA hairpinsfour-way junctionsHMGB1Intramolecular DNA junctionsnuclease resistancephosphorothioate bonds

Identifiers

PMID33342281
PMCPMC7988731
OpenAlexW3116395346

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.