Evidence map›Paper›PMID 33341231›Full record

ArticleFertility and sterility2021

Accelerated subcutaneous abdominal stem cell adipogenesis predicts insulin sensitivity in normal-weight women with polycystic ovary syndrome.

Daniel A Dumesic, Ayli Tulberg, Karen L Leung, Samantha C Fisch, Tristan R Grogan, David H Abbott, Rajanigandha Naik, Gregorio D Chazenbalk

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fertility and sterility, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed, 1 pooled it
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.

  1. Adipose Tissue Dysfunction in Polycystic Ovary Syndrome.The Journal of clinical endocrinology and metabolism · 2023
    Pooled it
  2. Trial
  3. Trial
  4. Article
  5. Article
  6. On the Intimate Relationship of Adiposity to Polycystic Ovary Syndrome.The Journal of clinical endocrinology and metabolism · 2025
    Review
  7. Review
  8. Article
  9. Review
  10. Shared Pathophysiological Mechanisms and Genetic Factors in Early Menarche and Polycystic Ovary Syndrome.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025
    Review
  11. Article
  12. Polycystic ovary syndrome.Nature reviews. Disease primers · 2024
    Review
  13. Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Daniel A DumesicDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California. Electronic address: ddumesic@mednet.ucla.edu.
Ayli TulbergDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California.
Karen L LeungDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California.
Samantha C FischDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California.
Tristan R GroganDepartment of Medicine Statistics Core, University of California, Los Angeles, Los Angeles, California.
David H AbbottOB/GYN, Wisconsin National Primate Research Center, University of Wisconsin, Madison, Madison, Wisconsin.
Rajanigandha NaikDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California.
Gregorio D ChazenbalkDepartment of Obstetrics and Gynecology, University of California, Los Angeles, Los Angeles, California.
University of California, Los Angeles · USUniversity of Wisconsin–Madison · US

Funding

Upgrade of confocal microscopy at the Oregon National Primate Research CenterP51OD011092 · OD · OREGON HEALTH & SCIENCE UNIVERSITY · PI Bonnie J. Nagel · 2012 to 2026
$203.9M
UCLA Clinical Translational Science InstituteUL1TR001881 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARLEEN F. BROWN, ARASH NAEIM · 2016 to 2026
$118.1M
UCLA Clinical and Translational Science InstituteUL1TR000124 · NCATS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DUBINETT, STEVEN M. · 2012 to 2015
$57.0M
PROJECT 4: ANDROGEN EXCESS IN ADIPOGENIC DYSFUNCTION IN PCOS WOMENP50HD071836 · NICHD · OREGON HEALTH & SCIENCE UNIVERSITY · PI DUMESIC, DANIEL A · 2014 to 2021
$14.7M
NCATS NIH HHS UL1 TR000124NCATS NIH HHS UL1 TR001881NICHD NIH HHS P50 HD071836NIH HHS P51 OD011092
6 · The paper itself

Abstract

objectiveTo examine whether subcutaneous (SC) abdominal adipose stem cell differentiation into adipocytes in vitro predicts insulin sensitivity (Si) in vivo in normal-weight women with polycystic ovary syndrome (PCOS) and controls.

designProspective cohort study.

settingAcademic medical center. PATIENT(S): Eight normal-weight women with PCOS and 8 age- and body mass index-matched controls. INTERVENTION(S): Women underwent circulating hormone/metabolic determinations, intravenous glucose tolerance testing, total-body dual-energy x-ray absorptiometry, and SC abdominal fat biopsy. MAIN OUTCOME MEASURE(S): PPARγ and CEBPa gene expression and lipid content of adipocytes matured in vitro were compared between women with PCOS and control women, and correlated with patient characteristics, systemic Si, and adipose insulin resistance (adipose-IR). RESULT(S): Serum androgen levels, adipose-IR, and percentage of android fat were greater in women with PCOS than control women. Stem cell PPARγ and CEBPa gene expression increased maximally by day 12 without a female-type effect. In control cells, gene expression positively correlated with fasting serum insulin levels (both genes) and adipose-IR (CEBPa) and negatively correlated with Si (CEBPa). Conversely, CEBPa gene expression in PCOS cells negatively correlated with adipose-IR and serum free testosterone, whereas total lipid accumulation in these cells positively corelated with Si.

conclusionIn normal-weight women with PCOS, accelerated SC abdominal adipose stem cell differentiation into adipocytes in vitro favors Si in vivo, suggesting a role for hyperandrogenism in the evolution of metabolic thrift to enhance fat storage through increased cellular glucose uptake.

Indexed as

AdipogenesisInsulin ResistanceAbdominal FatAdipocytesAdiposityAdultCase-Control StudiesCCAAT-Enhancer-Binding ProteinsCells, CulturedFemaleHumansHyperandrogenismIdeal Body WeightLipid MetabolismPolycystic Ovary SyndromePPAR gammaCCAAT-Enhancer-Binding ProteinsCEBPA protein, humanPPAR gammaPPARG protein, humanadipocyteAdiposehyperandrogenismpolycystic ovary syndromestem cell

Identifiers

PMID33341231
PMCPMC8209112
OpenAlexW3112632819

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.