Evidence map›Paper›PMID 33341069›Full record

ArticleBlood cells, molecules & diseases2021

Rasa3 regulates stage-specific cell cycle progression in murine erythropoiesis.

Elena C Brindley, Julien Papoin, Lauren Kennedy, Raymond F Robledo, Steven L Ciciotte, Theodosia A Kalfa, Luanne L Peters, Lionel Blanc

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Blood cells, molecules & diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Elena C BrindleyDepartment of Molecular Medicine and Pediatrics, Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY 11549, USA; Laboratory of Developmental Erythropoiesis, Les Nelkin Memorial Laboratory of Pediatric Oncology, Institute of Molecular Medicine, The Feinstein Institutes for Medical Research, Manhasset, NY 11030, USA.
Julien PapoinLaboratory of Developmental Erythropoiesis, Les Nelkin Memorial Laboratory of Pediatric Oncology, Institute of Molecular Medicine, The Feinstein Institutes for Medical Research, Manhasset, NY 11030, USA.
Lauren KennedyLaboratory of Developmental Erythropoiesis, Les Nelkin Memorial Laboratory of Pediatric Oncology, Institute of Molecular Medicine, The Feinstein Institutes for Medical Research, Manhasset, NY 11030, USA.
Raymond F RobledoThe Jackson Laboratory, Bar Harbor, ME 04672, USA.
Steven L CiciotteThe Jackson Laboratory, Bar Harbor, ME 04672, USA.
Theodosia A KalfaDepartment of Pediatrics, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 05229, USA.
Luanne L PetersThe Jackson Laboratory, Bar Harbor, ME 04672, USA.
Lionel BlancDepartment of Molecular Medicine and Pediatrics, Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY 11549, USA; Laboratory of Developmental Erythropoiesis, Les Nelkin Memorial Laboratory of Pediatric Oncology, Institute of Molecular Medicine, The Feinstein Institutes for Medical Research, Manhasset, NY 11030, USA. Electronic address: Lblanc@northwell.edu.
Feinstein Institute for Medical Research · USJackson Laboratory · USCincinnati Children's Hospital Medical Center · USHofstra University · US

Funding

Critical Regulatory Circuits in Blood FormationR01HL134043 · NHLBI · JACKSON LABORATORY · PI PETERS, LUANNE L · 2016 to 2019
$2.6M
Molecular targeting of erythroid progenitor cells in normal and disordered human erythropoiesisR01HL144436 · NHLBI · FEINSTEIN INSTITUTE FOR MEDICAL RESEARCH · PI BLANC, LIONEL · 2019 to 2023
$2.5M
The Role of Erythroblastic Islands in Anemia of InflammationR01HL152099 · NHLBI · CINCINNATI CHILDRENS HOSP MED CTR · PI BLANC, LIONEL, KALFA, THEODOSIA ANASTASIOS · 2020 to 2023
$2.5M
NHLBI NIH HHS R01 HL134043NHLBI NIH HHS R01 HL144436NHLBI NIH HHS R01 HL152099
6 · The paper itself

Abstract

Inherited bone marrow failure syndromes (IBMFS) are heterogeneous disorders characterized by dysregulated hematopoiesis in various lineages, developmental anomalies, and predisposition to malignancy. The scat (severe combined anemia and thrombocytopenia) mouse model is a model of IBMFS with a phenotype of pancytopenia cycling through crises and remission. Scat carries an autosomal recessive missense mutation in Rasa3 that results in RASA3 mislocalization and loss of function. RASA3 functions as a Ras-GTPase activating protein (GAP), and its loss of function in scat results in increased erythroid RAS activity and reactive oxygen species (ROS) and altered erythroid cell cycle progression, culminating in delayed terminal erythroid differentiation. Here we sought to further resolve the erythroid cell cycle defect in scat through ex vivo flow cytometric analyses. These studies revealed a specific G0/G1 accumulation in scat bone marrow (BM) polychromatophilic erythroblasts and scat BM Ter119

Indexed as

ErythropoiesisAnimalsCell CycleCells, CulturedErythroid CellsGTPase-Activating ProteinsMiceMice, Inbred BALB CMutation, Missenseras ProteinsGTPase-Activating ProteinsRASA3 protein, mouseras ProteinsCell cycleErythropoiesisInherited bone marrow failureRAS-GAPsRAS signaling

Identifiers

PMID33341069
PMCPMC7856249
OpenAlexW3113170041

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.