Evidence map›Paper›PMID 33340556›Full record

ArticleJournal of neuroscience methods2021

Transcranial chronic optical access to longitudinally measure cerebral blood flow.

Evelyn M Hoover, Christian Crouzet, Julianna M Bordas, Dario X Figueroa Velez, Sunil P Gandhi, Bernard Choi, Melissa B Lodoen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Evelyn M HooverUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, 92697, USA; University of California Irvine, Institute for Immunology, Irvine, 92697, USA.
Christian CrouzetUniversity of California Irvine, Department of Biomedical Engineering, Irvine, 92697, USA; University of California Irvine, Beckman Laser Institute and Medical Clinic, Irvine, 92697, USA.
Julianna M BordasUniversity of California Irvine, Department of Biomedical Engineering, Irvine, 92697, USA; University of California Irvine, Beckman Laser Institute and Medical Clinic, Irvine, 92697, USA.
Dario X Figueroa VelezUniversity of California Irvine, Department of Neurobiology and Behavior, Irvine, 92697, USA; University of California Irvine, Center for the Neurobiology of Learning and Memory, Irvine, 92697, USA.
Sunil P GandhiUniversity of California Irvine, Department of Neurobiology and Behavior, Irvine, 92697, USA; University of California Irvine, Center for the Neurobiology of Learning and Memory, Irvine, 92697, USA.
Bernard ChoiUniversity of California Irvine, Department of Biomedical Engineering, Irvine, 92697, USA; University of California Irvine, Beckman Laser Institute and Medical Clinic, Irvine, 92697, USA; University of California Irvine, Department of Surgery, Irvine, 92697, USA; University of California Irvine, Edwards Lifesciences Center for Advanced Cardiovascular Technology, Irvine, USA.
Melissa B LodoenUniversity of California Irvine, Department of Molecular Biology and Biochemistry, Irvine, 92697, USA; University of California Irvine, Institute for Immunology, Irvine, 92697, USA. Electronic address: mlodoen@uci.edu.
University of California, Irvine · USBeckman Laser Institute and Medical Clinic · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM008620 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI GOLDIN, ALAN L · 1999 to 2023
$8.1M
NRSA Training CoreTL1TR001415 · NCATS · UNIVERSITY OF CALIFORNIA-IRVINE · PI CAIOZZO, VINCENT JAMES, HEAD, ELIZABETH · 2015 to 2023
$4.2M
Reorganization of the Central Visual System by Inhibitory Neuron TransplantationR01EY029490 · NEI · UNIVERSITY OF CALIFORNIA-IRVINE · PI LUR, GYORGY · 2019 to 2023
$2.6M
Parasite dissemination in Toxoplasmic encephalitisR01AI120846 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI LODOEN, MELISSA BRUCKNER · 2016 to 2020
$2.0M
Immunology Research Training ProgramT32AI060573 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI PEARLMAN, ERIC · 2005 to 2020
$1.9M
Multiscale optical characterization of cerebrovascular structure, function and oxygen utilization in a novel mouse model of sporadic Alzheimer's diseaseR21AG066000 · NIA · UNIVERSITY OF CALIFORNIA-IRVINE · PI CHOI, BERNARD · 2020 to 2020
$411k
Neuroinflammation during cerebral Toxoplasma gondii infectionR21AI155122 · NIAID · UNIVERSITY OF CALIFORNIA-IRVINE · PI LODOEN, MELISSA BRUCKNER · 2021 to 2022
$395k
NCATS NIH HHS TL1 TR001415NEI NIH HHS R01 EY029490NIAID NIH HHS R01 AI120846NIAID NIH HHS R21 AI155122NIAID NIH HHS T32 AI060573NIA NIH HHS R21 AG066000NIGMS NIH HHS T32 GM008620
6 · The paper itself

Abstract

backgroundThe regulation of cerebral blood flow is critical for normal brain functioning, and many physiological and pathological conditions can have long-term impacts on cerebral blood flow. However, minimally invasive tools to study chronic changes in animal models are limited. NEW

methodWe developed a minimally invasive surgical technique (cyanoacrylate skull, CAS) allowing us to image cerebral blood flow longitudinally through the intact mouse skull using laser speckle imaging.

resultsWith CAS we were able to detect acute changes in cerebral blood flow induced by hypercapnic challenge. We were also able to image cerebral blood flow dynamics with laser speckle imaging for over 100 days. Furthermore, the relative cerebral blood flow remained stable in mice from 30 days to greater than 100 days after the surgery. COMPARISON WITH EXISTING

methodsPreviously, achieving continuous long-term optical access to measure cerebral blood flow in individual vessels in a mouse model involved invasive surgery. In contrast, the CAS technique presented here is relatively non-invasive, as it allows stable optical access through an intact mouse skull.

conclusionsThe CAS technique allows researcher to chronically measure cerebral blood flow dynamics for a significant portion of a mouse's lifespan. This approach may be useful for studying changes in blood flow due to cerebral pathology or for examining the therapeutic effects of modifying cerebral blood flow in mouse models relevant to human disease.

Indexed as

Cerebrovascular CirculationOptical ImagingAnimalsBrainHemodynamicsMiceSkullBrainCerebral blood flowCirculationIn vivo imagingLaser speckle imagingSurgeryVasculature

Identifiers

PMID33340556
PMCPMC8095351
OpenAlexW3110889398

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.