Engineering the Spatiotemporal Mosaic Self-Patterning of Pluripotent Stem Cells.

Ashley R G Libby, David A Joy, Todd C McDevitt

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Systems bioengineering approaches for developmental toxicology.Computational and structural biotechnology journal · 2023
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ashley R G LibbyDevelopmental and Stem Cell Biology PhD Program, University of California, San Francisco, CA, USA.

David A JoyGladstone Institute of Cardiovascular Disease, Gladstone Institutes, San Francisco, CA, USA.

Todd C McDevittGladstone Institute of Cardiovascular Disease, Gladstone Institutes, San Francisco, CA, USA. todd.mcdevitt@gladstone.ucsf.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pluripotent stem cells (PSCs) possess the ability to self-organize into complex tissue-like structures; however, the genetic mechanisms and multicellular dynamics that direct such patterning are difficult to control. Here, we pair live imaging with controlled induction of gene knockdown by CRISPR interference (CRISPRi) to generate changes within subpopulations of human PSCs, allowing for control over organization and analysis of emergent behaviors. Specifically, we use forced aggregation of mixtures of cells with and without an inducible CRISPRi system to knockdown molecular regulators of tissue symmetry. We then track the resulting multicellular organization through fluorescence live imaging concurrent with the induction of knockdown. Overall, this technique allows for controlled initiation of symmetry breaking by CRISPRi to produce changes in cellular behavior that can be tracked over time within high-density pluripotent stem cell colonies.

Indexed as

Body PatterningCRISPR-Cas SystemsGene EditingCells, CulturedClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Associated ProteinsGene Expression Regulation, DevelopmentalMicroscopy, FluorescenceMicroscopy, VideoPluripotent Stem CellsRNA, Guide, CRISPR-Cas SystemsSignal TransductionTime FactorsTime-Lapse ImagingCRISPR-Associated ProteinsRNA, Guide, CRISPR-Cas SystemsCell trackingCRISPR interferenceForced aggregationLive imagingMorphogenesisPluripotent stem cells

Identifiers

PMID33340357

DOI10.7717/peerj.453

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.