Evidence map›Paper›PMID 33340355›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2021

Engineering Biophysical Cues for Controlled 3D Differentiation of Endoderm Derivatives.

Thomas Richardson, Shibin Mathew, Connor Wiegand, Kevin Pietz, Joseph Candiello, K Ravikumar, Ipsita Banerjee

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Thomas RichardsonDepartment of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, USA.
Shibin MathewDepartment of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, USA.
Connor WiegandDepartment of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, USA.
Kevin PietzDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
Joseph CandielloDepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA.
K RavikumarDepartment of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, USA.
Ipsita BanerjeeDepartment of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, USA. ipb1@pitt.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Biophysical cues synergize with biochemical cues to drive differentiation of pluripotent stem cells through specific phenotypic trajectory. Tools to manipulate the cell biophysical environment and identify the influence of specific environment perturbation in the presence of combinatorial inputs will be critical to control the development trajectory. Here we describe the procedure to perturb biophysical environment of pluripotent stem cells while maintaining them in 3D culture configuration. We also discuss a high-throughput platform for combinatorial perturbation of the cell microenvironment, and detail a statistical procedure to extract dominant environmental influences.

Indexed as

Cell DifferentiationCell LineageFluorescent Antibody TechniqueMechanotransduction, CellularMicroscopy, FluorescenceStem Cell NicheTissue EngineeringAlginatesCell Culture TechniquesCells, CulturedEndodermGene Expression Regulation, DevelopmentalHumansModels, StatisticalPhenotypePluripotent Stem CellsAlginates3D Cell cultureAlginate encapsulationAlginate stiffnessBiophysical cueshPSC differentiation

Identifiers

PMID33340355

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.