ArticleThe Science of the total environment2021
Comparing analytical methods to detect SARS-CoV-2 in wastewater.
Article in The Science of the total environment, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers, 2 of them syntheses that pooled it.
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Who cites it
89 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- A Systematic Review of Methodological Approaches to SARS-CoV-2 Wastewater Surveillance.Viruses · 2026Pooled it
- Recent progress on wastewater-based epidemiology for COVID-19 surveillance: A systematic review of analytical procedures and epidemiological modeling.The Science of the total environment · 2023Pooled it
- Evaluating Viral Pollution in Wastewater and Mediterranean Ecosystems.Food and environmental virology · 2026Article
- Designing effective SARS-CoV-2 wastewater surveillance system: lessons learnt from sustained monitoring in Delhi, India.BMC infectious diseases · 2026Article
- Comparative Analysis of Concentration and Quantification Methods for Antibiotic Resistance Genes and Their Phage-Mediated Dissemination in Treated Wastewater and Biosolids.Pathogens (Basel, Switzerland) · 2025Article
- Variabilities in N2 and E Gene Concentrations in a SARS-CoV-2 Wastewater Multiplex Assay.Microorganisms · 2025Article
- Insights Into Pandemic and Post-Pandemic Dynamics of Enteric Viruses in a Middle-Size City-Burgos, Spain-Using a Long-Term Wastewater Surveillance.Food and environmental virology · 2025Article
- Assess the Variability and Robustness of an Aluminum-Based Adsorption-Precipitation Method for Virus Detection in Wastewater Samples.Microorganisms · 2025Article
- Presence of Potentially Infectious Human Enteric Viruses and Antibiotic Resistance Genes in Mussels from the Campania Region, Italy: Implications for Consumer's Safety.Food and environmental virology · 2025Article
- The potential of long-term wastewater-based surveillance to predict COVID-19 waves peak in Mexico.Water environment research : a research publication of the Water Environment Federation · 2025Article
- The Impact of the Variability of RT-qPCR Standard Curves on Reliable Viral Detection in Wastewater Surveillance.Microorganisms · 2025Article
- Comprehensive Study of Antibiotics and Antibiotic Resistance Genes in Wastewater and Impacted Mediterranean Water Environments.Antibiotics (Basel, Switzerland) · 2025Article
- The influence of environmental factors on the detection and quantification of SARS-CoV-2 variants in dormitory wastewater at a primarily undergraduate institution.Microbiology spectrum · 2025Article
- Optimized Aluminum Hydroxide Adsorption-Precipitation for Improved Viral Detection in Wastewater.International journal of environmental research and public health · 2025Article
- The Impact of Viral Concentration Method on Quantification and Long Amplicon Nanopore Sequencing of SARS-CoV-2 and Noroviruses in Wastewater.Microorganisms · 2025Article
- Consistent trends from different methods for monitoring SARS-CoV-2 in urban wastewater during a 29-month longitudinal study.Frontiers in microbiology · 2025Article
- Compact highly sensitive photothermal RT-LAMP chip for simultaneous multidisease detection.Science advances · 2024Article
- A Narrative Review of High Throughput Wastewater Sample Processing for Infectious Disease Surveillance: Challenges, Progress, and Future Opportunities.International journal of environmental research and public health · 2024Review
- Tracking epidemic viruses in wastewaters.Microbial biotechnology · 2024Review
- Inter-institutional laboratory standardization for SARS-CoV-2 surveillance through wastewater-based epidemiology applied to Mexico City.IJID regions · 2024Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wastewater based epidemiology (WBE) has emerged as a reliable strategy to assess the coronavirus disease 2019 (COVID-19) pandemic. Recent publications suggest that SARS-CoV-2 detection in wastewater is technically feasible; however, many different protocols are available and most of the methods applied have not been properly validated. To this end, different procedures to concentrate and extract inactivated SARS-CoV-2 and surrogates were initially evaluated. Urban wastewater seeded with gamma-irradiated SARS-CoV-2, porcine epidemic diarrhea virus (PEDV), and mengovirus (MgV) was used to test the concentration efficiency of an aluminum-based adsorption-precipitation method and a polyethylene glycol (PEG) precipitation protocol. Moreover, two different RNA extraction methods were compared in this study: a commercial manual spin column centrifugation kit and an automated protocol based on magnetic silica beads. Overall, the evaluated concentration methods did not impact the recovery of gamma-irradiated SARS-CoV-2 nor MgV, while extraction methods showed significant differences for PEDV. Mean recovery rates of 42.9 ± 9.5%, 27.5 ± 14.3% and 9.0 ± 2.2% were obtained for gamma-irradiated SARS-CoV-2, PEDV and MgV, respectively. Limits of detection (LoD
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Registered trials
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