Evidence map›Paper›PMID 33338433›Full record

ArticleStem cell reports2021

Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos.

Chi-Hun Park, Young-Hee Jeoung, Kyung-Jun Uh, Ki-Eun Park, Jessica Bridge, Anne Powell, Jie Li, Laramie Pence, Luhui Zhang, Tianbin Liu and 6 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 20 citations in OpenAlex.

  1. Review
  2. Functional Inactivation ofInternational journal of molecular sciences · 2026
    Article
  3. Insulin-like growth factor 1 receptor (Frontiers in cell and developmental biology · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Current Status of Synthetic Mammalian Embryo Models.International journal of molecular sciences · 2024
    Review
  9. Article
  10. Article
  11. Article
  12. Pigs lackingFrontiers in genome editing · 2023
    Article
  13. Review
  14. Limited Expansion of Human Hepatocytes in FAH/Tissue engineering. Part A · 2022
    Article
  15. Article
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 7 institutions in 2 countries.

Chi-Hun ParkAnimal and Avian Sciences, University of Maryland, College Park, MD 20742, USA; Animal Bioscience and Biotechnology Laboratory, USDA, ARS, Beltsville, MD 20705, USA. Electronic address: chpark@umd.edu.
Young-Hee JeoungAnimal and Avian Sciences, University of Maryland, College Park, MD 20742, USA; Animal Bioscience and Biotechnology Laboratory, USDA, ARS, Beltsville, MD 20705, USA.
Kyung-Jun UhDepartment of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA.
Ki-Eun ParkAnimal and Avian Sciences, University of Maryland, College Park, MD 20742, USA; Animal Bioscience and Biotechnology Laboratory, USDA, ARS, Beltsville, MD 20705, USA; RenOVAte Biosciences Inc, Reisterstown, MD 21136, USA.
Jessica BridgeAnimal and Avian Sciences, University of Maryland, College Park, MD 20742, USA; Animal Bioscience and Biotechnology Laboratory, USDA, ARS, Beltsville, MD 20705, USA.
Anne PowellAnimal Bioscience and Biotechnology Laboratory, USDA, ARS, Beltsville, MD 20705, USA; RenOVAte Biosciences Inc, Reisterstown, MD 21136, USA.
Jie LiBGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China; BGI-Shenzhen, Shenzhen, 518083, China; Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen, 518120, China; Guangdong Provincial Academician Workstation of BGI Synthetic Genomics, BGI-Shenzhen, Guangdong, China.
Laramie PenceAnimal and Avian Sciences, University of Maryland, College Park, MD 20742, USA; Animal Bioscience and Biotechnology Laboratory, USDA, ARS, Beltsville, MD 20705, USA.
Luhui ZhangAnimal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.
Tianbin LiuBGI-Shenzhen, Shenzhen, 518083, China; Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen, 518120, China; Guangdong Provincial Academician Workstation of BGI Synthetic Genomics, BGI-Shenzhen, Guangdong, China.
Hai-Xi SunBGI-Shenzhen, Shenzhen, 518083, China; Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen, 518120, China; Guangdong Provincial Academician Workstation of BGI Synthetic Genomics, BGI-Shenzhen, Guangdong, China.
Ying GuBGI-Shenzhen, Shenzhen, 518083, China; Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen, 518120, China; Guangdong Provincial Academician Workstation of BGI Synthetic Genomics, BGI-Shenzhen, Guangdong, China.
Yue ShenBGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China; BGI-Shenzhen, Shenzhen, 518083, China; Guangdong Provincial Key Laboratory of Genome Read and Write, Shenzhen, 518120, China; Guangdong Provincial Academician Workstation of BGI Synthetic Genomics, BGI-Shenzhen, Guangdong, China; Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics, Shenzhen, 518120, China.
Jun WuDepartment of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA; Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Juan-Carlos Izpisua BelmonteSalk Institute, La Jolla, San Diego, CA 92037, USA.
Bhanu P TeluguAnimal and Avian Sciences, University of Maryland, College Park, MD 20742, USA; Animal Bioscience and Biotechnology Laboratory, USDA, ARS, Beltsville, MD 20705, USA; RenOVAte Biosciences Inc, Reisterstown, MD 21136, USA. Electronic address: btelugu@umd.edu.
BGI Group (China) · CNBeltsville Agricultural Research Center · USUniversity of Maryland, College Park · USBioScience Laboratories (United States) · USSalk Institute for Biological Studies · USThe University of Texas Southwestern Medical Center · USVirginia Tech · US

Funding

A porcine model for investigating the role of an insulin signaling regulator in development and diseaseR01HD092304 · NICHD · UNIV OF MARYLAND, COLLEGE PARK · PI SALEM, MOHAMED · 2018 to 2022
$1.6M
NICHD NIH HHS R01 HD092304
6 · The paper itself

Abstract

Most of our current knowledge regarding early lineage specification and embryo-derived stem cells comes from studies in rodent models. However, key gaps remain in our understanding of these developmental processes from nonrodent species. Here, we report the detailed characterization of pig extraembryonic endoderm (pXEN) cells, which can be reliably and reproducibly generated from primitive endoderm (PrE) of blastocyst. Highly expandable pXEN cells express canonical PrE markers and transcriptionally resemble rodent XENs. The pXEN cells contribute both to extraembryonic tissues including visceral yolk sac as well as embryonic gut when injected into host blastocysts, and generate live offspring when used as a nuclear donor in somatic cell nuclear transfer (SCNT). The pXEN cell lines provide a novel model for studying lineage segregation, as well as a source for genome editing in livestock.

Indexed as

AnimalsBlastocystCell LineageChimeraCollagen Type I, alpha 1 ChainCRISPR-Cas SystemsEmbryo, MammalianEndodermGene EditingMiceSwineCollagen Type I, alpha 1 ChainCloningembryonic chimeraextra-embryonic cellsgut endodermpig/porcineprimitive endodermSCNTstem cellXENyolk sac

Identifiers

PMID33338433
PMCPMC7897585
OpenAlexW3112847690

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.