ArticleRevista da Sociedade Brasileira de Medicina Tropical2020
Filter paper performance in PCR for cutaneous leishmaniasis diagnosis.
Article in Revista da Sociedade Brasileira de Medicina Tropical, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
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Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 11 citations in OpenAlex.
- A novel pan-Leishmania loop-mediated isothermal amplification (Loopamp) assay for diagnosis of cutaneous and visceral leishmaniasis: a systematic review and meta-analysis.BMC infectious diseases · 2025Pooled it
- Ketoconazole for Cutaneous Leishmaniasis in the Context of Drug Shortages: A Case Report from Madre de Dios, Peru.The Yale journal of biology and medicine · 2025Article
- Ongoing presence of Leishmania major cutaneous leishmaniasis in Mauritania, 2016-2024.BMC infectious diseases · 2025Article
- Evaluation of Loopamp Leishmania detection kit for the diagnosis of cutaneous leishmaniasis in Ethiopia.Parasites & vectors · 2024Article
- The Use of Dried Matrix Spots as an Alternative Sampling Technique for Monitoring Neglected Tropical Diseases.Pathogens (Basel, Switzerland) · 2024Review
- Sampling is decisive to determination of Leishmania (Viannia) species.PLoS neglected tropical diseases · 2024Article
- Article
- Diagnostic accuracy of qPCR and microscopy for cutaneous leishmaniasis in rural Ecuador: A Bayesian latent class analysis.PLoS neglected tropical diseases · 2023Article
- Dried Blood Spots (DBS): A suitable alternative to using whole blood samples for diagnostic testing of visceral leishmaniasis in the post-elimination era.PLoS neglected tropical diseases · 2023Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis.
methodsLesion scrapings from 48 patients were collected and analyzed for PCR.
resultsPCR with FP detected up to three Leishmania braziliensis promastigotes. Considering the direct search by microscopy or PCR of samples collected in STE buffer as standards, the sensitivity of PCR with FP was 100%.
conclusionsFP can be useful for CL diagnosis in remote regions, allowing high sensitivity in the detection of the parasite by PCR.
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Registered trials
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