ArticleiScience2020
Multicolor 3D-dSTORM Reveals Native-State Ultrastructure of Polysaccharides' Network during Plant Cell Wall Assembly.
Article in iScience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Single-molecule localization microscopy imaging of extracellular vesicle DNA in recipient cells.Journal of translational medicine · 2026Article
- Stiffness transitions in new walls post-cell division differ betweenProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Disruption of a DUF247 Containing Protein Alters Cell Wall Polysaccharides and Reduces Growth inPlants (Basel, Switzerland) · 2023Article
- Article
- Spatial proteomics of vesicular trafficking: coupling mass spectrometry and imaging approaches in membrane biology.Plant biotechnology journal · 2023Review
- The role of pectin phase separation in plant cell wall assembly and growth.Cell surface (Amsterdam, Netherlands) · 2021Review
- Protocol for multicolor three-dimensional dSTORM data analysis using MATLAB-based script package Grafeo.STAR protocols · 2021Article
- Current and future advances in fluorescence-based visualization of plant cell wall components and cell wall biosynthetic machineries.Biotechnology for biofuels · 2021Review
- From monocots to dicots: the multifold aspect of cell wall expansion.Journal of experimental botany · 2021Article
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Authors and funding
3 authors.
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Abstract
The plant cell wall, a form of the extracellular matrix, is a complex and dynamic network of polymers mediating a plethora of physiological functions. How polysaccharides assemble into a coherent and heterogeneous matrix remains mostly undefined. Further progress requires improved molecular-level visualization methods that would gain a deeper understanding of the cell wall nanoarchitecture. dSTORM, a type of super-resolution microscopy, permits quantitative nanoimaging of the cell wall. However, due to the lack of single-cell model systems and the requirement of tissue-level imaging, its use in plant science is almost absent. Here we overcome these limitations; we compare two methods to achieve three-dimensional dSTORM and identify optimal photoswitching dyes for tissue-level multicolor nanoscopy. Combining dSTORM with spatial statistics, we reveal and characterize the ultrastructure of three major polysaccharides, callose, mannan, and cellulose, in the plant cell wall precursor and provide evidence for cellulose structural re-organization related to callose content.
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