Evidence map›Paper›PMID 33336096›Full record

ArticleHeliyon2020

A comparison of exosome purification methods using serum of Marek's disease virus (MDV)-vaccinated and -tumor-bearing chickens.

Sabari Nath Neerukonda, Nicholas A Egan, Joseph Patria, Imane Assakhi, Phaedra Tavlarides-Hontz, Shannon Modla, Eric R Muñoz, Matthew B Hudson, Mark S Parcells

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Article
  2. Exosome isolation based on polyethylene glycol (PEG): a review.Molecular and cellular biochemistry · 2025
    Review
  3. MnMikrochimica acta · 2023
    Article
  4. Review
  5. Principles and Problems of Exosome Isolation from Biological Fluids.Biochemistry (Moscow) Supplement. Series A, Membrane and cell biology · 2022
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Sabari Nath NeerukondaDepartment of Animal and Food Sciences, University of Delaware, Newark, DE 19716, USA.
Nicholas A EganDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA.
Joseph PatriaDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA.
Imane AssakhiDepartment of Biological Sciences, University of Delaware, Newark, DE 19716, USA.
Phaedra Tavlarides-HontzDepartment of Animal and Food Sciences, University of Delaware, Newark, DE 19716, USA.
Shannon ModlaDelaware Biotechnology Institute, Bioimaging Center, Newark, DE 19711, USA.
Eric R MuñozDepartment of Kinesiology and Applied Physiology, University of Delaware, Newark, DE 19716, USA.
Matthew B HudsonDepartment of Kinesiology and Applied Physiology, University of Delaware, Newark, DE 19716, USA.
Mark S ParcellsDepartment of Animal and Food Sciences, University of Delaware, Newark, DE 19716, USA.
University of Delaware · USBiotechnology Institute · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Marek's disease (MD) is an alphaherpesvirus (Marek's disease virus, MDV)-induced pathology of chickens associated with paralysis, immunosuppression, neurological signs, and T-cell lymphomas. MD is controlled in poultry production via live attenuated vaccines. The purpose of the current study was to compare methods for precipitating exosomes from vaccinated and protected chicken sera (VEX) and tumor-bearing chicken sera (TEX) for biomarker analysis of vaccine-induced protection and MD lymphomas respectively. A standard polyethylene glycol (PEG, 8%) method was compared to a commercial reagent (total exosome isolation reagent, TEI) for exosome yield and RNA content. Although exosomes purified by PEG or TEI were comparable in size and morphology, TEI-reagent yielded 3-4-fold greater concentration. Relative expression of 8 out of 10

Indexed as

Animal scienceExosome precipitationExtracellular vesicleHematological systemImmunologyMarek's disease virusmiRNATranscriptomicsVeterinary medicineVirology

Identifiers

PMID33336096
PMCPMC7734234
OpenAlexW3112463374

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.