Evidence map›Paper›PMID 33335751›Full record

ArticleTranslational neuroscience2020

Hydrochloride fasudil attenuates brain injury in ICH rats.

Limin Li, Xiaoli Lou, Kunlun Zhang, Fangping Yu, Yingchun Zhao, Ping Jiang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational neuroscience, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Neuroprotective Strategies in Aneurysmal Subarachnoid Hemorrhage (aSAH).International journal of molecular sciences · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Limin LiDepartment of Neurology, The Affiliated Shanghai Songjiang Central Hospital of Shanghai Jiao Tong University, Central Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Xiaoli LouDepartment of Neurology, The Affiliated Shanghai Songjiang Central Hospital of Shanghai Jiao Tong University, Central Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Kunlun ZhangDepartment of Neurology, The Affiliated Shanghai Songjiang Central Hospital of Shanghai Jiao Tong University, Central Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Fangping YuDepartment of Neurology, The Affiliated Shanghai Songjiang Central Hospital of Shanghai Jiao Tong University, Central Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yingchun ZhaoDepartment of Neurology, The Affiliated Shanghai Songjiang Central Hospital of Shanghai Jiao Tong University, Central Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Ping JiangDepartment of Neurology, The Affiliated Shanghai Songjiang Central Hospital of Shanghai Jiao Tong University, Central Hospital of Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shanghai Jiao Tong University · CNSongjiang District Central Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimThe aim of this study was to investigate the neuroprotective effects of hydrochloride fasudil (HF) in rats following intracerebral hemorrhage (ICH).

methodsMale Wistar rats were randomly divided into four groups: normal, sham-operated, ICH, and ICH/HF. ICH was induced by injection of non-anticoagulant autologous arterial blood into the right caudate nucleus. The levels of Rho-associated protein kinase 2 (ROCK2) mRNA and protein around the site of the hematoma were measured by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay (ELISA), respectively. The levels of interleukin-6 and tumor necrosis factor-α in serum were detected by ELISA. The inflammatory cells and changes in the neuronal morphology around the hematoma were visualized using hematoxylin and eosin and Nissl staining. Brain edema was measured by comparing wet and dry brain weights.

resultsFollowing ICH, the levels of ROCK2 were significantly increased from day 1 to day 7. The levels of ROCK2 were significantly lower in rats treated with HF than in controls. The levels of inflammatory cytokines and brain water content were significantly higher in rats treated with HF than in controls. Administration of HF significantly reduced the levels of inflammatory cytokines and brain water content from day 1 to day 7. In the acute phase of ICH, a large number of neutrophils infiltrated the perihematomal areas. In comparison with the ICH group, the ICH/HF group showed markedly fewer infiltrating neutrophils on day 1. Nissl staining showed that ICH caused neuronal death and loss of neurons in the perihematomal areas at all time points and that treatment with HF significantly attenuated neuronal loss.

conclusionsHF exerts neuroprotective effects in ICH rats by inhibiting the expression of ROCK2, reducing neutrophil infiltration and production of inflammatory cytokines, decreasing brain edema, and attenuating loss of neurons.

Indexed as

brain injuryhydrochloride fasudilinflammationintracerebral hemorrhageRho-associated protein kinase

Identifiers

PMID33335751
PMCPMC7712513
OpenAlexW3033124956

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.