ArticleScientific reports2020
siRNA biogenesis and advances in topically applied dsRNA for controlling virus infections in tomato plants.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 56 citations in OpenAlex.
- Cucurbit Leaf Crumple Virus: An Important Pathogen of Cucurbit and Snap Bean Crops.Molecular plant pathology · 2026Review
- The unfulfilled potential of nanocarriers for RNA delivery in antiviral crop protection.Nature plants · 2026Review
- Control Strategies of Plant Viruses Using Spray-Induced Gene Silencing.The plant pathology journal · 2026Review
- Control of tomato brown rugose fruit virus (ToBRFV) in tomato plants using in vivo synthesized dsRNA.Journal of experimental botany · 2026Article
- Carbon Dots and mesoporous silica nanocomposites improve spray-induced gene silencing to suppress plant RNA and DNA viruses.Scientific reports · 2026Article
- Hairpin-RNA spray confers resistance to mungbean yellow mosaic India virus in mungbean.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Morphology effect of a novel biocompatible nucleic acid delivery nanosystem of g-CPlant biotechnology journal · 2025Article
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Integrating RNA Interference and Nanotechnology: A Transformative Approach in Plant Protection.Plants (Basel, Switzerland) · 2025Review
- Spray-induced gene silencing for crop protection: recent advances and emerging trends.Frontiers in plant science · 2025Review
- Review
- RNA interference: a promising biotechnological approach to combat plant pathogens, mechanism and future prospects.World journal of microbiology & biotechnology · 2024Review
- Comparative analysis of RNA interference and pattern-triggered immunity induced by dsRNA reveals different efficiencies in the antiviral response to potato virus X.Molecular plant pathology · 2024Article
- Detection of exogenous siRNA inside sweet corn bundle sheath cells and the RNAi dynamics in the early stage ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Development of Attenuated Viruses for Effective Protection against Pepper Veinal Mottle Virus in Tomato Crops.Viruses · 2024Article
- Exogenous Application of dsRNA for Protection against Tomato Leaf Curl New Delhi Virus.Viruses · 2024Article
- A Non-Canonical Pathway Induced by Externally Applied Virus-Specific dsRNA in Potato Plants.International journal of molecular sciences · 2023Article
- Exogenous delivery of dsRNA for management of mungbean yellow mosaic virus on blackgram.Planta · 2023Article
- Analysis of Virus-Derived siRNAs in Strawberry Plants Co-Infected with Multiple Viruses and Their Genotypes.Plants (Basel, Switzerland) · 2023Article
- Silencing ofInsects · 2022Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A non-transgenic approach based on RNA interference was employed to induce protection against tomato mosaic virus (ToMV) infection in tomato plants. dsRNA molecules targeting the cp gene of ToMV were topically applied on plants prior to virus inoculation. Protection was dose-dependent and sequence-specific. While no protection was achieved when 0-16 µg dsRNA were used, maximum rates of resistance (60 and 63%) were observed in doses of 200 and 400 µg/plant, respectively. Similar rates were also obtained against potato virus Y when targeting its cp gene. The protection was quickly activated upon dsRNA application and lasted for up to 4 days. In contrast, no detectable antiviral response was triggered by the dsRNA from a begomovirus genome, suggesting the method is not effective against phloem-limited DNA viruses. Deep sequencing was performed to analyze the biogenesis of siRNA populations. Although long-dsRNA remained in the treated leaves for at least 10 days, its systemic movement was not observed. Conversely, dsRNA-derived siRNA populations (mainly 21- and 22-nt) were detected in non-treated leaves, which indicates endogenous processing and transport through the plant. Altogether, this study provides critical information for the development of novel tools against plant viruses; strengths and limitations inherent to the systems are discussed.
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