ArticleScientific reports2020
Molecular detection of Mycobacterium tuberculosis from buccal swabs among adult in Peru.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 3 of them syntheses that pooled it.
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Who cites it
17 citing papers in PubMed, 3 syntheses or guidelines pooled it, 18 citations in OpenAlex.
- Oral swabs with a rapid molecular diagnostic test for pulmonary tuberculosis in adults and children: a systematic review.The Lancet. Global health · 2024Pooled it
- Accuracy of upper respiratory tract samples to diagnose Mycobacterium tuberculosis: a systematic review and meta-analysis.The Lancet. Microbe · 2023Pooled it
- Diagnostic accuracy of oral swab for detection of pulmonary tuberculosis: a systematic review and meta-analysis.Frontiers in medicine · 2023Pooled it
- Developmental validation of the rapidpuncher, an automated laser-based sampling system that eliminates cross-contamination in the forensic analysis of FTA cards.International journal of legal medicine · 2026Article
- Oral Rinse as an Alternative Diagnostic Specimen for Detection of Tuberculosis.American journal of respiratory and critical care medicine · 2025Article
- A DNA Methylation Signature From Buccal Swabs to Identify Tuberculosis Infection.The Journal of infectious diseases · 2025Article
- Early health technology assessment of tongue swab for non-sputum based pulmonary tuberculosis diagnosis in Thailand.The Lancet regional health. Southeast Asia · 2025Article
- A DNA methylation signature identified in the buccal mucosa reflecting active tuberculosis is changing during tuberculosis treatment.Scientific reports · 2024Article
- Non-sputum-based samples and biomarkers for detection of Mycobacterium tuberculosis: the hope to improve childhood and HIV-associated tuberculosis diagnosis.European journal of medical research · 2024Review
- Detection of Mycobacterium tuberculosis transrenal DNA in urine samples among adults in Peru.Tuberculosis (Edinburgh, Scotland) · 2024Article
- Blazing the trail for innovative tuberculosis diagnostics.Infection · 2024Review
- Leptin and Leptin Receptor Polymorphisms in Infants and Their Parents: Correlation with Preterm Birth.Genes · 2024Article
- Diagnostic Yield of Nucleic Acid Amplification Tests in Oral Samples for Pulmonary Tuberculosis: A Systematic Review and Meta-analysis.Open forum infectious diseases · 2023Review
- The performance of tongue swabs for detection of pulmonary tuberculosis.Frontiers in cellular and infection microbiology · 2023Article
- Oral Swab Specimens Tested With Xpert MTB/RIF Ultra Assay for Diagnosis of Pulmonary Tuberculosis in Children: A Diagnostic Accuracy Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2022Article
- Diagnostic performance of patient self-collected oral swab (tongue and cheek) in comparison with healthcare worker-collected nasopharyngeal swab for severe acute respiratory syndrome coronavirus-2 detection.APMIS : acta pathologica, microbiologica, et immunologica Scandinavica · 2022Article
- Bioarchaeological and palaeogenomic portrait of two Pompeians that died during the eruption of Vesuvius in 79 AD.Scientific reports · 2022Article
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tuberculosis (TB) diagnosis relies on a sputum sample, which cannot be easily obtained from all symptomatic patients. Mycobacterium tuberculosis DNA can be detected from oral swabs, a noninvasive, safe alternative sample type; however, reported sensitivities have been variable and likely depend on sample collection, processing procedures and host characteristics. We analyzed three buccal swab samples from 123 adults with culture-confirmed TB in Lima, Peru. We compared the sensitivity and specificity of two sample collection devices (OmniSwab and EasiCollect FTA cards) and examined factors associated with detection. DNA was extracted with a commercially available kit and detected via real-time PCR IS6110 amplification. Overall sensitivity for buccal samples was 51% (95% Confidence Interval [CI] 42-60%). Specificity from a single sample among healthy controls was 96.7% (95% CI 83-99.9%). Positive sputum smear and cavitary disease, correlates of disease burden, were associated with detection via buccal swab. Although we observed higher sensitivities with the Omniswab samples, this appeared to be due primarily to differences in patient characteristics (e.g., cavitary disease). Overall, our findings support the potential for a buccal sample-based TB assay. Future work should focus on assay optimization and streamlining the assay workflow.
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