ArticleScientific reports2020
Selection and validation of reference genes for quantitative gene expression normalization in Taxus spp.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 23 citations in OpenAlex.
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- TcJAMYC5 positively regulates paclitaxel biosynthesis in Taxus chinensis var. Mairei.Plant molecular biology · 2025Article
- qRT-PCR Reference Gene Selection for the Discoloration of Tender Leaves in Hawk Tea (Current issues in molecular biology · 2025Article
- Selection and validation of reference genes for qRT-PCR normalization in dayflower (Commelina communis) based on the transcriptome profiling.BMC plant biology · 2024Article
- Identification and validation of qRT-PCR reference genes for analyzing grape infection with gray mold.BMC genomics · 2024Article
- Reference Gene Selection for RT-qPCR Normalization inInternational journal of molecular sciences · 2024Article
- Identification of the Optimal Quantitative RT-PCR Reference Gene for Paper Mulberry (Current issues in molecular biology · 2024Article
- Reference Genes Screening and Gene Expression Patterns Analysis Involved in Gelsenicine Biosynthesis under Different Hormone Treatments inInternational journal of molecular sciences · 2023Article
- Screening and validating of endogenous reference genes in Chlorella sp. TLD 6B under abiotic stress.Scientific reports · 2023Article
- Reference gene identification for normalisation of RT-qPCR analysis in plasma samples of the rat middle cerebral artery occlusion model.Veterinary medicine and science · 2022Article
- A Selection of Reliable Reference Genes for Gene Expression Analysis in the Female and Male Flowers ofPlants (Basel, Switzerland) · 2022Article
- TcMYB29a, an ABA-Responsive R2R3-MYB Transcriptional Factor, Upregulates Taxol Biosynthesis inFrontiers in plant science · 2022Article
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Authors and funding
8 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Quantitative real-time PCR (qRT-PCR) is commonly used to measure gene expression to further explore gene function, while suitable reference genes must be stably expressed under different experimental conditions to obtain accurate and reproducible data for relative quantification. Taxol or paclitaxel is an important anticancer compound mainly identified in Taxus spp. The molecular mechanism of the regulation of taxol biosynthesis is current research goal. However, in the case of Taxus spp., few reports were published on screening suitable reference genes as internal controls for qRT-PCR. Here, eight reference genes were selected as candidate reference genes for further study. Common statistical algorithms geNorm, NormFinder, BestKeeper, ΔCt, and RefFinder were used to analyze the data from samples collected from a cell line of Taxus × media under various experimental conditions and from tissues of Taxus chinensis var. mairei. The expression patterns of TcMYC under salicylic acid treatment differed significantly, with the best and worst reference genes in the cell line. This study screened out suitable reference genes (GAPDH1 and SAND) under different treatments and tissues for the accurate and reliable normalization of the qRT-PCR expression data of Taxus spp. At the same time, this study will aid future research on taxol biosynthesis-related genes expression in Taxus spp., and can also be directly used to other related species.
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