ArticleScientific reports2020
A combined 3D printing/CNC micro-milling method to fabricate a large-scale microfluidic device with the small size 3D architectures: an application for tumor spheroid production.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 64 citations in OpenAlex.
- Development of a Microwell System for Reproducible Formation of Homogeneous Cell Spheroids.Pharmaceutics · 2025Article
- A Protocol Guide to Micro Milling for Bio-Microfluidics.Bio-protocol · 2025Article
- Augmented 3D Printing for Multiscale Microphysiological Systems.Small (Weinheim an der Bergstrasse, Germany) · 2025Review
- Review
- Microinjection molded microwell array-based portable digital PCR system for the detection of infectious respiratory viruses.Nano convergence · 2025Article
- Extrusion bioprinting: meeting the promise of human tissue biofabrication?Progress in biomedical engineering (Bristol, England) · 2025Review
- Microfluidic Monodispersed Microbubble Generation for Production of Cavitation Nuclei.Micromachines · 2024Article
- Machine Learning-Driven Innovations in Microfluidics.Biosensors · 2024Review
- A Thorough Review of Emerging Technologies in Micro- and Nanochannel Fabrication: Limitations, Applications, and Comparison.Micromachines · 2024Review
- Automated Uniform Spheroid Generation Platform for High Throughput Drug Screening Process.Biosensors · 2024Article
- Heterotypic tumor spheroids: a platform for nanomedicine evaluation.Journal of nanobiotechnology · 2023Review
- Microfluidics and Organoids, the Power Couple of Developmental Biology and Oncology Studies.International journal of molecular sciences · 2023Review
- On the Application of Microfluidic-Based Technologies in Forensics: A Review.Sensors (Basel, Switzerland) · 2023Review
- Spheroid Engineering in Microfluidic Devices.ACS omega · 2023Review
- Recent advances in biological pumps as a building block for bioartificial hearts.Frontiers in bioengineering and biotechnology · 2023Review
- Fabrication of Concave Microwells and Their Applications in Micro-Tissue Engineering: A Review.Micromachines · 2022Review
- Fluid Viscosity Measurement by Means of Secondary Flow in a Curved Channel.Micromachines · 2022Article
- An insight into the potentials of carbon dots for in vitro live-cell imaging: recent progress, challenges, and prospects.Mikrochimica acta · 2022Review
- 3D-bioprinted cancer-on-a-chip: level-up organotypic in vitro models.Trends in biotechnology · 2022Review
- PlateFlo - A software-controllable plate-scale perfusion system for culture of adherent cells.HardwareX · 2021Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fabrication of a large-scale microfluidic mold with 3D microstructures for manufacturing of the conical microwell chip using a combined projection micro-stereolithography (PµSL) 3D printing/CNC micro-milling method for tumor spheroid formation is presented. The PµSL technique is known as the most promising method of manufacturing microfluidic chips due to the possibility of creating complex three-dimensional microstructures with high resolution in the range of several micrometers. The purpose of applying the proposed method is to investigate the influence of microwell depths on the formation of tumor spheroids. In the conventional methods, the construction of three-dimensional microstructures and multi-height chips is difficult, time-consuming, and is performed using a multi-step lithography process. Microwell depth is an essential parameter for microwell design since it directly affects the shear stress of the fluid flow and the diffusion of nutrients, respiratory gases, and growth factors. In this study, a chip was made with microwells of different depth varying from 100 to 500 µm. The mold of the microwell section is printed by the lab-made PµSL printer with 6 and 1 µm lateral and vertical resolutions. Other parts of the mold, such as the main chamber and micro-channels, were manufactured using the CNC micro-milling method. Finally, different parts of the master mold were assembled and used for PDMS casting. The proposed technique drastically simplifies the fabrication and rapid prototyping of large-scale microfluidic devices with high-resolution microstructures by combining 3D printing with the CNC micro-milling method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.