Evidence map›Paper›PMID 33333020›Full record

ArticleCell2020

Generation of Functional Human 3D Cortico-Motor Assembloids.

Jimena Andersen, Omer Revah, Yuki Miura, Nicholas Thom, Neal D Amin, Kevin W Kelley, Mandeep Singh, Xiaoyu Chen, Mayuri Vijay Thete, Elisabeth M Walczak and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 328 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
328citing papers in PubMed, 2 pooled it
25.9field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

328 citing papers in PubMed, 2 syntheses or guidelines pooled it, 525 citations in OpenAlex.

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268 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Jimena AndersenDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA; Stanford Brain Organogenesis Program, Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA 94305, USA.
Omer RevahDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA; Stanford Brain Organogenesis Program, Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA 94305, USA.
Yuki MiuraDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA; Stanford Brain Organogenesis Program, Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA 94305, USA.
Nicholas ThomDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA.
Neal D AminDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA; Stanford Brain Organogenesis Program, Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA 94305, USA.
Kevin W KelleyDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA; Stanford Brain Organogenesis Program, Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA 94305, USA.
Mandeep SinghDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA; Stanford Brain Organogenesis Program, Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA 94305, USA.
Xiaoyu ChenDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA; Stanford Brain Organogenesis Program, Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA 94305, USA.
Mayuri Vijay TheteDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA.
Elisabeth M WalczakBD Biosciences, 4040 Campbell Ave Suite 110, Menlo Park, CA 94025, USA.
Hannes VogelDepartments of Pathology and Pediatrics, Stanford University, Stanford, CA 94305, USA.
H Christina FanBD Biosciences, 4040 Campbell Ave Suite 110, Menlo Park, CA 94025, USA.
Sergiu P PaşcaDepartment of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA 94305, USA; Stanford Brain Organogenesis Program, Wu Tsai Neurosciences Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: spasca@stanford.edu.
Neurosciences Institute · USStanford University · USBD Biosciences (United States) · US

Funding

Gaining insight into psychiatric disease by engineering piece by piece the human brain in vitro.R01MH107800 · NIMH · STANFORD UNIVERSITY · PI PASCA, SERGIU · 2015 to 2024
$5.6M
Transmission Electron MicroscopeS10RR026780 · NCRR · STANFORD UNIVERSITY · PI TALBOT, WILLIAM S · 2010 to 2010
$412k
NCRR NIH HHS S10 RR026780NIMH NIH HHS R01 MH107800
6 · The paper itself

Abstract

Neurons in the cerebral cortex connect through descending pathways to hindbrain and spinal cord to activate muscle and generate movement. Although components of this pathway have been previously generated and studied in vitro, the assembly of this multi-synaptic circuit has not yet been achieved with human cells. Here, we derive organoids resembling the cerebral cortex or the hindbrain/spinal cord and assemble them with human skeletal muscle spheroids to generate 3D cortico-motor assembloids. Using rabies tracing, calcium imaging, and patch-clamp recordings, we show that corticofugal neurons project and connect with spinal spheroids, while spinal-derived motor neurons connect with muscle. Glutamate uncaging or optogenetic stimulation of cortical spheroids triggers robust contraction of 3D muscle, and assembloids are morphologically and functionally intact for up to 10 weeks post-fusion. Together, this system highlights the remarkable self-assembly capacity of 3D cultures to form functional circuits that could be used to understand development and disease.

Indexed as

AnimalsCalciumCell DifferentiationCells, CulturedCerebral CortexCervical VertebraeGene Expression RegulationGlutamatesHumansInduced Pluripotent Stem CellsMiceMotor CortexMusclesMyoblastsNerve NetOptogeneticsCalciumGlutamatesassembloidscerebral cortexconnectivitycorticospinalhuman pluripotent stem cellsneuromuscularoptogeneticsorganoidsrabies tracingspinal cord

Identifiers

PMID33333020
PMCPMC8711252
OpenAlexW3111725926

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.