ArticleFEMS yeast research2021
Metabolic engineering of Saccharomyces cerevisiae for production of β-carotene from hydrophobic substrates.
Article in FEMS yeast research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 42 citations in OpenAlex.
- Integrating Machine Learning and Transcriptomics to Enhance β‑Carotene Production inACS omega · 2026Article
- Bioethanol from Lignocellulosic Feedstock: with Different Co-Cultivation Strategies.Iranian journal of biotechnology · 2026Review
- CRISPR/Cas9-Mediated Metabolic Engineering of EndophyticACS omega · 2026Article
- Development and application of synthetic biology tools for improved production of human breast milk components.Synthetic and systems biotechnology · 2025Review
- Sustainable β-carotene production by engineered S. cerevisiae using sucrose and agricultural by-products.Bioresources and bioprocessing · 2025Article
- Review
- Two-Phase Fermentation Systems for Microbial Production of Plant-Derived Terpenes.Molecules (Basel, Switzerland) · 2024Review
- Microbial Pigments: Major Groups and Industrial Applications.Microorganisms · 2023Review
- Industrially Important Fungal Carotenoids: Advancements in Biotechnological Production and Extraction.Journal of fungi (Basel, Switzerland) · 2023Review
- Status of food colorants in India: conflicts and prospects.Journal fur Verbraucherschutz und Lebensmittelsicherheit = Journal of consumer protection and food safety · 2023Review
- Carotenoids and Their Biosynthesis in Fungi.Molecules (Basel, Switzerland) · 2022Review
- Recent advances in construction and regulation of yeast cell factories.World journal of microbiology & biotechnology · 2022Review
- Biotechnological advances for improving natural pigment production: a state-of-the-art review.Bioresources and bioprocessing · 2022Review
- Toward improved terpenoids biosynthesis: strategies to enhance the capabilities of cell factories.Bioresources and bioprocessing · 2022Review
- Carotenoid metabolism: New insights and synthetic approaches.Frontiers in plant science · 2022Review
- The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts.Frontiers in microbiology · 2022Review
- Carotenoids and Some Other Pigments from Fungi and Yeasts.Metabolites · 2021Review
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
β-Carotene is a yellow-orange-red pigment used in food, cosmetics and pharmacy. There is no commercial yeast-based process for β-carotene manufacturing. In this work, we engineered the baker's yeast Saccharomyces cerevisiae by expression of lipases and carotenogenic genes to enable the production of β-carotene on hydrophobic substrates. First, the extracellular lipase (LIP2) and two cell-bound lipases (LIP7 and LIP8) from oleaginous yeast Yarrowia lipolytica were expressed either individually or in combination in S. cerevisiae. The engineered strains could grow on olive oil and triolein as the sole carbon source. The strain expressing all three lipases had ∼40% lipid content per dry weight. Next, we integrated the genes encoding β-carotene biosynthetic pathway, crtI, crtYB and crtE from Xanthophyllomyces dendrorhous. The resulting engineered strain bearing the lipases and carotenogenic genes reached a titer of 477.9 mg/L β-carotene in yeast peptone dextrose (YPD) medium supplemented with 1% (v/v) olive oil, which was 12-fold higher than an analogous strain without lipases. The highest β-carotene content of 46.5 mg/g DCW was obtained in yeast nitrogen base (YNB) medium supplemented with 1% (v/v) olive oil. The study demonstrates the potential of applying lipases and hydrophobic substrate supplementation for the production of carotenoids in S. cerevisiae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.