ArticlePLoS neglected tropical diseases2020
Genetic diversity of Trypanosoma cruzi parasites infecting dogs in southern Louisiana sheds light on parasite transmission cycles and serological diagnostic performance.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Vampire bats in Belize harbor multiple Trypanosoma cruzi genotypes: Implications for parasite transmission at the wildlife-domestic-human interface.PLoS neglected tropical diseases · 2026Article
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- Beyond Pilot Data: Reframing Tc-24 ELISA in the Diagnostic Ecology of Canine Chagas Disease.Veterinary medicine and science · 2026Article
- A critical review of American Trypanosomiasis (AT) in dogs with the current distribution of cases in Brazil and a diagnostic approach for veterinary clinicians.Veterinary research communications · 2026Review
- Evaluation of a Novel Tc-24 Recombinant Antigen ELISA for Serologic Detection of Trypanosoma cruzi Infection in Dogs: A Pilot Study.Veterinary medicine and science · 2025Article
- Trypanosoma cruzi DTU TcI disproportionately associated with fatal T. cruzi myocarditis in dogs in Texas, USA.Veterinary parasitology, regional studies and reports · 2025Article
- Characterizing the transmission dynamics of Trypanosoma cruzi in Triatoma sanguisuga collected from dog kennels in southern Texas.Parasites & vectors · 2025Article
- Coinfection by multipleMicrobiology and molecular biology reviews : MMBR · 2025Review
- Natural infection of Trypanosoma Cruzi in client-owned-dogs from rural Yucatan, Mexico.Scientific reports · 2025Article
- Triatoma venosa and Panstrongylus geniculatus challenge the certification of interruption of vectorial Trypanosoma cruzi transmission by Rhodnius prolixus in eastern Colombia.PLoS neglected tropical diseases · 2025Article
- Metabarcoding study of potential pathogens and zoonotic risks associated with dog feces in Seoul, South Korea.PLoS neglected tropical diseases · 2024Article
- Genetic Diversity ofLife (Basel, Switzerland) · 2024Review
- Evaluation of chimeric recombinant antigens for the serodiagnosis of Trypanosoma cruzi in dogs: a promising tool for Chagas disease surveillance.Parasites & vectors · 2024Article
- Chagas Disease: A Silent Threat for Dogs and Humans.International journal of molecular sciences · 2024Review
- New insights intoFrontiers in immunology · 2024Review
- Intra-hostMicrobiology spectrum · 2023Article
- Exclusion of Horizontal and Vertical Transmission as Major Sources ofComparative medicine · 2023Article
- Article
- Molecular detection ofParasitology · 2022Article
- Geographic Variations in Test Reactivity for the Serological Diagnosis of Trypanosoma cruzi Infection.Journal of clinical microbiology · 2021Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundChagas disease is a neglected zoonosis of growing concern in the southern US, caused by the parasite Trypanosoma cruzi. We genotyped parasites in a large cohort of PCR positive dogs to shed light on parasite transmission cycles and assess potential relationships between parasite diversity and serological test performance. METHODOLOGY/PRINCIPAL
findingsWe used a metabarcoding approach based on deep sequencing of T. cruzi mini-exon marker to assess parasite diversity. Phylogenetic analysis of 178 sequences from 40 dogs confirmed the presence of T. cruzi discrete typing unit (DTU) TcI and TcIV, as well as TcII, TcV and TcVI for the first time in US dogs. Infections with multiple DTUs occurred in 38% of the dogs. These data indicate a greater genetic diversity of T. cruzi than previously detected in the US. Comparison of T. cruzi sequence diversity indicated that highly similar T. cruzi strains from these DTUs circulate in hosts and vectors in Louisiana, indicating that they are involved in a shared T. cruzi parasite transmission cycle. However, TcIV and TcV were sampled more frequently in vectors, while TcII and TcVI were sampled more frequently in dogs. CONCLUSIONS/SIGNIFICANCE: These observations point to ecological host-fitting being a dominant mechanism involved in the diversification of T. cruzi-host associations. Dogs with negative, discordant or confirmed positive T. cruzi serology harbored TcI parasites with different mini-exon sequences, which strongly supports the hypothesis that parasite genetic diversity is a key factor affecting serological test performance. Thus, the identification of conserved parasite antigens should be a high priority for the improvement of current serological tests.
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