ArticleNucleic acids research2021
Structural basis of sequestration of the anti-Shine-Dalgarno sequence in the Bacteroidetes ribosome.
Article in Nucleic acids research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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24 citing papers in PubMed, 42 citations in OpenAlex.
- Deep learning-drivenSynthetic and systems biotechnology · 2027Article
- Regulation of multiple paralogs of a small subunit ribosomal protein inRNA (New York, N.Y.) · 2026Article
- Role of the anti-Shine-Dalgarno sequence of 16S rRNA in Flavobacterium johnsoniae.Nucleic acids research · 2026Article
- Structure of a specialized 70S initiation complex.Nucleic acids research · 2026Article
- Extended Shine-Dalgarno motifs govern translation initiation in Staphylococcus aureus.Nature communications · 2026Article
- Structural studies of ribosome from an anaerobic Bacteroidetes human pathogen Porphyromonas gingivalis.Nucleic acids research · 2025Article
- Protein bL38 facilitates incorporation of uL6 during assembly of the 50S subunit in Flavobacterium johnsoniae.Nucleic acids research · 2025Article
- Structures of Saccharolobus solfataricus initiation complexes with leaderless mRNAs highlight archaeal features and eukaryotic proximity.Nature communications · 2025Article
- Genetic landscape of primary mitochondrial diseases in children and adults using molecular genetics and genomic investigations of mitochondrial and nuclear genome.Orphanet journal of rare diseases · 2024Article
- Extraribosomal Functions of Bacterial Ribosomal Proteins-An Update, 2023.International journal of molecular sciences · 2024Review
- Analysis of programmed frameshifting during translation ofRNA (New York, N.Y.) · 2024Article
- Unraveling the plasticity of translation initiation in prokaryotes: Beyond the invariant Shine-Dalgarno sequence.PloS one · 2024Article
- The structure of a hibernating ribosome in a Lyme disease pathogen.Nature communications · 2023Article
- Interactions between terminal ribosomal RNA helices stabilize theRNA (New York, N.Y.) · 2023Article
- Ribosome heterogeneity results in leader sequence-mediated regulation of protein synthesis inJournal of bacteriology · 2023Article
- Initiator AUGs Are Discriminated from Elongator AUGs Predominantly through mRNA Accessibility in C. crescentus.Journal of bacteriology · 2023Article
- Sarecycline inhibits protein translation in Cutibacterium acnes 70S ribosome using a two-site mechanism.Nucleic acids research · 2023Article
- Ribosomes lacking bS21 gain function to regulate protein synthesis in Flavobacterium johnsoniae.Nucleic acids research · 2023Article
- Article
- A Ribosomal Protein Homolog Governs Gene Expression and Virulence in a Bacterial Pathogen.Journal of bacteriology · 2022Article
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11 authors at 2 institutions in 2 countries.
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Abstract
Genomic studies have indicated that certain bacterial lineages such as the Bacteroidetes lack Shine-Dalgarno (SD) sequences, and yet with few exceptions ribosomes of these organisms carry the canonical anti-SD (ASD) sequence. Here, we show that ribosomes purified from Flavobacterium johnsoniae, a representative of the Bacteroidetes, fail to recognize the SD sequence of mRNA in vitro. A cryo-electron microscopy structure of the complete 70S ribosome from F. johnsoniae at 2.8 Å resolution reveals that the ASD is sequestered by ribosomal proteins bS21, bS18 and bS6, explaining the basis of ASD inhibition. The structure also uncovers a novel ribosomal protein-bL38. Remarkably, in F. johnsoniae and many other Flavobacteriia, the gene encoding bS21 contains a strong SD, unlike virtually all other genes. A subset of Flavobacteriia have an alternative ASD, and in these organisms the fully complementary sequence lies upstream of the bS21 gene, indicative of natural covariation. In other Bacteroidetes classes, strong SDs are frequently found upstream of the genes for bS21 and/or bS18. We propose that these SDs are used as regulatory elements, enabling bS21 and bS18 to translationally control their own production.
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