ArticleFrontiers in cell and developmental biology2020
Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells.
Article in Frontiers in cell and developmental biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 31 citations in OpenAlex.
- The ER-Resident Protein PDZD8 Inhibits Virus Production of West Nile Virus.Microbiology and immunology · 2026Article
- Spatial architecture of immunometabolism: Mitochondrial‑organelle interfaces in immune signaling (Review).International journal of molecular medicine · 2026Review
- Advanced imaging of membrane contact sites.Nature cell biology · 2026Review
- Heterogeneity, dynamics and organelle interactions of lipid droplets.Nature reviews. Molecular cell biology · 2026Review
- The spatiotemporal dynamics of MAMs: mechanisms, pathologies, and therapeutic rewiring.Cellular & molecular biology letters · 2026Review
- A guide to characterizing the dynamic mitochondria-endoplasmic reticulum contact sites.The FEBS journal · 2025Review
- Split genetically encoded calcium indicators for interorganellar junctions.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Imaging interorganelle contacts at a glance.Journal of cell science · 2024Review
- Small GTPase ActIvitY ANalyzing (SAIYAN) system: A method to detect GTPase activation in living cells.The Journal of cell biology · 2024Article
- A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.The Journal of cell biology · 2024Article
- Organelle landscape analysis using a multiparametric particle-based method.PLoS biology · 2024Article
- Emerging Designs and Applications for Biomembrane Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2024Review
- Imaging and proteomics toolkits for studying organelle contact sites.Frontiers in cell and developmental biology · 2024Review
- A fluorogenic complementation tool kit for interrogating lipid droplet-organelle interaction.bioRxiv : the preprint server for biology · 2023Article
- Article
- Extended-Synaptotagmin 1 Enhances Liver Cancer Progression Mediated by the Unconventional Secretion of Cytosolic Proteins.Molecules (Basel, Switzerland) · 2023Article
- Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission.Journal of cell science · 2023Article
- Proximity-Ligation Assay to Detect Peroxisome-Organelle Interaction.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Dissociation of ERMES clusters plays a key role in attenuating the endoplasmic reticulum stress.iScience · 2022Article
- Systematic analysis of membrane contact sites ineLife · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Inter-organelle contact sites have attracted a lot of attention as functionally specialized regions that mediate the exchange of metabolites, including lipids and ions, between distinct organelles. However, studies on inter-organelle contact sites are at an early stage and it remains enigmatic what directly mediates the organelle-organelle interactions and how the number and degree of the contacts are regulated. As a first step to answer these questions, we previously developed split-GFP probes that could visualize and quantify multiple inter-organelle contact sites in the yeast and human cultured cells. However, the split-GFP probes possessed a disadvantage of inducing artificial connections between two different organelle membranes, especially when overexpressed. In the present study, we developed a way to express the split-GFP probes whose expressions remained at low levels, with minimal variations between different yeast cells. Besides, we constructed a HeLa cell line in which the expression of the split-GFP probes could be induced by the addition of doxycycline to minimize the artificial effects. The improved split-GFP systems may be faithful tools to quantify organelle contact sites and screen new factors involved in organelle-organelle tethering in yeast and mammalian cells.
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